Phellodendron chinense Schneid is an important Chinese herb with berberine and phellodendrine in stems and leaves, but with little information available on in vitro culture of this species. Disinfection of explants in 75% alcohol for 45 s, sterilization in 0.1% HgCl₂ for 20 min, and submersion in 1.0 mol L⁻¹ gibberellin3 (GA₃) solution for 24 h was the optimal condition for seed germination. Murashige and Skoog’s (MS) medium supplemented with 2.0 mg L⁻¹ 6-benzylaminopurine (6-BA) in combination with 1.5 mg L⁻¹ 1-naphthylacetic acid (NAA) was optimal for callus induction. MS medium supplemented with 2.0 mg L⁻¹ 6-BA was the appropriate medium for induction of adventitious shoots, and 1/2MS medium supplemented with 2.0 mg L⁻¹ indole-3-butytric acid (IBA) and 0.5% active carbon was the optimal medium for root induction. The 15-d survival rate of regenerated plantlets after transplanting to basins containing perlite and peat moss (1:4) was greater than 80%, and the berberine and phellodendrine accumulation was lower in callus compared with regenerated plantlets. The establishment of highly efficient regeneration system provides technical support for genetic breeding of Phellodendron chinense Schneid.

黄柏是一种重要的中草药，茎和叶中含有小ber碱和黄柏碱，但该种的体外培养信息很少。外植体在75％酒精中消毒45 s，在0.1％HgCl _2中消毒20分钟，然后浸入1.0 mol L ^-1赤霉素₃（GA ₃）溶液中24 h是种子发芽的最佳条件。补充有2.0 mg L ^-1 6-苄氨基嘌呤（6-BA）和1.5 mg L ^-1 1-萘乙酸（NAA）的Murashige和Skoog's（MS）培养基最适合诱导愈伤组织。MS培养基中添加了2.0 mg L ^-16-BA是诱导不定芽的合适培养基，补充有2.0 mg L ^-1吲哚-3-丁酸（IBA）和0.5％活性炭的1 / 2MS培养基是诱导根的最佳培养基。移植到含有珍珠岩和泥炭苔藓（1：4）的盆地后，再生苗的15天存活率大于80％，与再生苗相比，愈伤组织中的小ber碱和黄柏树素积累较低。高效再生系统的建立为黄柏遗传育种提供了技术支持。