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Metatranscriptomic analysis of modified atmosphere packaged poultry meat enables prediction of Brochothrix thermosphacta and Carnobacterium divergens in situ metabolism
Archives of Microbiology ( IF 2.8 ) Pub Date : 2020-05-27 , DOI: 10.1007/s00203-020-01914-y
Linda Höll 1 , Maik Hilgarth 1 , Andreas J Geissler 1 , Jürgen Behr 1, 2 , Rudi F Vogel 1
Affiliation  

In this study, in situ-expressed metabolic routes of Brochothrix (B.) thermosphacta and Carnobacterium (C.) divergens were evaluated based on a metatranscriptomic dataset from bacteria growing on MAP chicken meat (O 2 /CO 2 ; N 2 /CO 2 ). Both species exhibited no ( C. divergens ) or minor transcription regulation ( B. thermosphacta ) within their main metabolic routes in response to different atmospheres. Both employ pathways related to glucose and ribose. Gluconeogenesis from lipid-borne glycerol is active in the progressing lack of carbohydrates. Pyruvate fates in both species comprise lactate, ethanol, acetate, CO 2 , formate, C4-compounds and H 2 O 2 (only B. thermosphacta ). Both species express genes for a minimal aerobic respiratory chain, but do not possess the genetic setting for a functional citric acid cycle. While products of carbohydrate and glycerol metabolism display mild to medium sensorial off-characteristics, predicted end products of their amino acid metabolism comprise, e.g., isobutyrate and isovalerate ( B. thermosphacta ) or cadaverine and tyramine ( C. divergens ) as potent spoilage compounds.

中文翻译:

气调包装家禽肉的元转录组学分析能够预测 Brochothrix thermosphacta 和 Carnobacterium divergens 原位代谢

在本研究中,基于来自生长在 MAP 鸡肉上的细菌的宏转录组数据集(O 2 /CO 2 ;N 2 /CO 2 )评估了 Brochothrix (B.) thermosphacta 和 Carnobacterium (C.) divergens 的原位表达代谢途径)。这两个物种在响应不同大气的主要代谢途径中没有表现出(C.divergens)或轻微的转录调节(B.thermosphacta)。两者都采用与葡萄糖和核糖相关的途径。脂类甘油的糖异生在碳水化合物的逐渐缺乏中很活跃。两种物种中的丙酮酸归宿包括乳酸盐、乙醇、乙酸盐、CO 2 、甲酸盐、C4-化合物和 H 2 O 2 (仅 B.thermosphacta)。两个物种都表达最小有氧呼吸链的基因,但不具有功能性柠檬酸循环的遗传环境。
更新日期:2020-05-27
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