Antibiotic residues in foods have aroused wide public concern because of their potential side-effects. It is imperative to develop a simple, accurate and reliable method for the detection of antibiotic residues in foods. In this paper, we report a novel, facile and sensitive method for the detection of ampicillin in milk using a commercial personal glucose meter (PGM). Magnetic beads (MBs) were employed as the platform, an ampicillin aptamer was used as the recognition element and streptavidin was utilized as the bridge to link invertase and the aptamer. After the hydrolysis of sucrose to glucose, the concentration of glucose was quantitatively measured using the PGM. The difference of PGM signals with and without addition of ampicillin exhibits a good linear correlation with the logarithm of ampicillin concentrations in the range of 2.5 × 10⁻¹⁰ mol L⁻¹ to 1.0 × 10⁻⁷ mol L⁻¹ with a detection limit of 2.5 × 10⁻¹⁰ mol L⁻¹ (S/N = 3). Finally, the proposed method was successfully applied for the detection of ampicillin residue in milk.

中文翻译：

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使用个人血糖仪检测牛奶中氨苄青霉素的适体传感器。

食品中的抗生素残留物由于其潜在的副作用而引起了广泛的关注。必须开发一种简单，准确和可靠的方法来检测食品中的抗生素残留。在本文中，我们报告了一种使用商用个人血糖仪（PGM）检测牛奶中氨苄青霉素的新颖，简便而灵敏的方法。磁珠（MBs）被用作平台，氨苄青霉素适体被用作识别元件，而链霉亲和素被用作连接转化酶和适体的桥。蔗糖水解成葡萄糖后，使用PGM定量测量葡萄糖的浓度。添加和不添加氨苄青霉素的PGM信号的差异与氨苄青霉素浓度的对数在2.5×10范围内表现出良好的线性相关性^-10 mol L ^-1至1.0×10 ^-7 mol L ^-1，检测极限为2.5×10 ^-10 mol L ^-1（S / N = 3）。最终，该方法成功应用于牛奶中氨苄西林残留的检测。