We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs/group): negative control (NC); wild-type PRV inoculation (PRV 3CR Ossabaw; hereafter PRV); PRV vaccination (Ingelvac Aujeszky MLV; Boehringer Ingelheim; hereafter MLV); and PRV vaccination followed by PRV inoculation at 21 d post-vaccination (MLV-PRV). Using a serum PRV whole-virus indirect IgG ELISA (Idexx Laboratories) adapted to the oral fluid matrix, PRV antibody was detected in oral fluid samples from treatment groups PRV, MLV, and MLV-PRV in a pattern similar to serum. Vaccination alone produced a low oral fluid antibody response (groups MLV and MLV-PRV), but a strong anamnestic response was observed following challenge with wild-type virus (group PRV). Analyses of the oral fluid PRV indirect IgG ELISA results showed good binary diagnostic performance (area under ROC curve = 93%) and excellent assay repeatability (intra-class correlation coefficient = 99.3%). The demonstrable presence of PRV antibodies in swine oral fluids suggests the possible use of oral fluids in pseudorabies surveillance.

我们评估了猪口腔液中伪狂犬病病毒（PRV）抗体的检测。从分配给4个治疗组的40头猪（10只猪/组）中获得口服液和血清样品：阴性对照（NC）；阴性对照（NC）。野生型PRV接种（PRV 3CR Ossabaw；此后称为PRV）；PRV疫苗接种（Ingelvac Aujeszky MLV; Boehringer Ingelheim;以下简称MLV）；接种后21 d（MLV-PRV）接种PRV疫苗，然后接种PRV。使用适合口腔液基质的血清PRV全病毒间接IgG ELISA（Idexx Laboratories），以类似于血清的模式在治疗组PRV，MLV和MLV-PRV的口腔液样品中检测到PRV抗体。单独接种疫苗产生的口服液抗体反应较低（MLV和MLV-PRV组），但在野生型病毒（PRV组）攻击后观察到了强烈的记忆消除反应。口服液PRV间接IgG ELISA结果的分析显示出良好的二元诊断性能（ROC曲线下的面积= 93％）和出色的测定重复性（类内相关系数= 99.3％）。猪口服液中PRV抗体的明显存在表明在伪狂犬病监测中可能使用了口服液。