Crosslinking mass spectrometry is widening its scope from structural analyzes of purified multi-protein complexes towards systems-wide analyzes of protein-protein interactions. Assessing the error in these large datasets is currently a challenge. Using a controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate a reliable false-discovery rate estimation procedure for protein-protein interactions identified by crosslinking mass spectrometry.

中文翻译：

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通过交联质谱法可靠地鉴定蛋白质间相互作用

交联质谱法的范围已从纯化的多蛋白复合物的结构分析扩展到蛋​​白-蛋白相互作用的全系统分析。目前，评估这些大型数据集中的错误是一个挑战。使用大肠杆菌细胞裂解物的受控大规模分析，我们证明了通过交联质谱法鉴定的蛋白质-蛋白质相互作用的可靠的错误发现率估算程序。