The GlycReSoft software tool allows users to process glycoproteomics LC-MS data sets. The tool accepts proteomics database search results or a user-defined list of proteins in the sample. GlycReSoft processes LC-MS data to yield deconvoluted exact mass values. The user has the option to import a list of theoretical glycans from an external database, a curated glycan list, or a measured glycome. The tool assembles a list of theoretical glycopeptides from the lists of theoretical glycans and proteins, respectively. The program then scores the tandem mass spectra in the LC-MS data files and provides graphical views of the identified glycopeptides for each protein in the sample, and the set of glycoforms identified for each peptide sequence.

中文翻译：

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使用GlycReSoft分析糖蛋白组学LC-MS数据的协议

GlycReSoft软件工具允许用户处理糖蛋白组学LC-MS数据集。该工具接受蛋白质组学数据库的搜索结果或样品中用户定义的蛋白质列表。GlycReSoft处理LC-MS数据以产生去卷积的精确质量值。用户可以选择从外部数据库中导入理论聚糖列表，经过整理的聚糖列表或测得的糖组。该工具分别从理论聚糖和蛋白质列表中组装出理论糖肽列表。然后，该程序对LC-MS数据文件中的串联质谱进行评分，并提供样品中每种蛋白质的鉴定糖肽以及每种肽序列鉴定的糖型组的图形视图。