Autophagosome biogenesis is a dynamic membrane event, which is executed by the sequential function of autophagy-related (ATG) proteins. Upon autophagy induction, a cup-shaped membrane structure appears in the cytoplasm, then elongates sequestering cytoplasmic materials, and finally forms a closed double membrane autophagosome. However, how this complex vesicle formation event is strictly controlled and achieved is still enigmatic. Recently, there is accumulating evidence showing that some ATG proteins have the ability to directly interact with membranes, transfer lipids between membranes and regulate lipid metabolism. A novel role for various membrane lipids in autophagosome formation is also emerging. Here, we highlight past and recent key findings on the function of ATG proteins related to autophagosome biogenesis and consider how ATG proteins control this dynamic membrane formation event to organize the autophagosome by collaborating with membrane lipids.

自噬体生物发生是一个动态的膜事件，由自噬相关（ATG）蛋白的顺序功能执行。自噬诱导后，细胞质中出现杯状膜结构，然后拉长隔离的细胞质物质，最后形成封闭的双膜自噬体。然而，如何严格控制和实现这种复杂的囊泡形成事件仍然是个谜。最近，越来越多的证据表明，一些ATG蛋白具有直接与膜相互作用、在膜之间转移脂质和调节脂质代谢的能力。各种膜脂在自噬体形成中的新作用也正在出现。这里，