This study was conducted to determine whether nature-derived Reynoutria elliptica extracts exhibit biocompatibility and antimicrobial effects against oral pathogens such as Streptococcus mutans and Candida albicans. Fine particles of Reynoutria elliptica extract were used to probe for biocompatibility and antimicrobial activity toward these pathogens, and results were evaluated with an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, spectrophotometric growth inhibitory assay, the total number of colony-forming units (CFU), an agar disk diffusion test, and scanning electron microscopy (SEM). In addition, UV/VIS spectroscopy was used to determine the levels of flavonoid and polyphenol in experimental solutions. Several experimental groups showed cell viability higher than 70%, and the antimicrobial activity toward both S. mutans and C. albicans was significantly higher than was that seen for the control group. In CFU and agar disk diffusion tests with C. albicans, increases in the concentration of Reynoutria elliptica extract led to significantly increased antimicrobial effects. Additionally, SEM results showed that Reynoutria elliptica extract changed the morphology and density of S. mutans and C. albicans. The results of this research can be applied to the use of Reynoutria elliptica extracts for the development of oral products that are biologically friendly and can control oral diseases such as dental caries and candida-associated denture stomatitis.

中文翻译：

---

椭圆白花提取物的生物相容性和抗菌活性。

进行了这项研究以确定自然来源的椭圆形Reynoutria椭圆形提取物是否表现出对口腔病原体例如变形链球菌和白色念珠菌的生物相容性和抗菌作用。用椭圆藻提取物的细小颗粒探测这些病原体的生物相容性和抗菌活性，并用MTT（3- [4,5-二甲基噻唑-2-基] -2,5二苯基溴化四氮唑）测定，分光光度法生长抑制测定，菌落形成单位总数（CFU），琼脂磁盘扩散测试和扫描电子显微镜（SEM）。另外，使用UV / VIS光谱法测定实验溶液中的类黄酮和多酚含量。几个实验组显示细胞活力高于70％，并且对变形链球菌和白色念珠菌的抗菌活性均显着高于对照组。在用白色念珠菌进行CFU和琼脂圆盘扩散试验中，椭圆形雷氏菌的浓度增加提取物导致抗菌效果显着提高。另外，扫描电镜结果表明，椭圆叶提取物改变了变形链球菌和白色念珠菌的形态和密度。这项研究的结果可用于将Reynoutria elliptica提取物用于口腔友好产品的开发，该产品具有生物学上的优势并可以控制口腔疾病，例如龋齿和念珠菌相关的假牙口腔炎。