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Retinal defocus and form-deprivation induced regional differential gene expression of bone morphogenetic proteins in chick retinal pigment epithelium.
The Journal of Comparative Neurology ( IF 2.5 ) Pub Date : 2020-05-26 , DOI: 10.1002/cne.24957
Yan Zhang 1 , Sara Azmoun 1 , Abraham Hang 1 , Jiexi Zeng 2 , Emily Eng 1 , Christine F Wildsoet 1
Affiliation  

We previously reported bidirectional gene expression regulation of the Bone Morphogenetic Proteins (BMP2, 4, and 7) in chick retinal pigment epithelium (RPE) in response to imposed optical defocus and form‐deprivation (FD). This study investigated whether there are local (regional) differences in these effects. 19‐day old White‐Leghorn chicks wore monocular +10 or − 10 D lenses, or diffusers (FD) for 2 or 48 hr, after which RPE samples were collected from both eyes, from a central circular zone (3 mm radius), and 3 mm wide annular mid‐peripheral and peripheral zones in all cases. BMP2, 4, and 7 gene expression levels in RPE from treated and fellow control eyes were compared as well as differences across zones. With the +10 D lens, increased expression of both BMP2 and BMP4 genes was observed in central and mid‐peripheral zones but not the peripheral zone after 2 and 48 hr. In contrast, with the −10 D lens BMP2 gene expression was significantly decreased in all three zones after 2 and 48 hr. Similar patterns of BMP2 gene expression were observed in all three zones after 48 hr of FD. Smaller changes were recorded for BMP4 and BMP7 gene expression for both myopia‐inducing treatments. That optical defocus‐ and FD‐induced changes in BMP gene expression in chick RPE show treatment‐dependent local (regional) differences suggest important differences in the nature and contributions of local retinal and underlying RPE regions to eye growth regulation.

中文翻译:

视网膜离焦和形式剥夺诱导鸡视网膜色素上皮中骨形态发生蛋白的区域差异基因表达。

我们以前报道的骨形态发生蛋白的双向基因表达调控(BMP24,和7响应于施加的光学散焦和形觉剥夺(FD))在小鸡视网膜色素上皮细胞(RPE)。本研究调查了这些影响是否存在局部(区域)差异。19 天大的 White-Leghorn 雏鸡佩戴单眼 +10 或 - 10 D 镜片或扩散器 (FD) 2 或 48 小时,然后从中央圆形区域(半径 3 毫米)收集双眼 RPE 样本,在所有情况下,3 毫米宽的环形中周边和周边区域。BMP24,和7比较了治疗眼和其他对照眼的 RPE 中的基因表达水平以及区域间的差异。使用 +10 D 镜片,在 2 小时和 48 小时后,在中央和中周边区域观察到BMP2BMP4基因的表达增加,但在周边区域未观察到。相比之下,使用 -10 D 晶状体,在 2 小时和 48 小时后,所有三个区域的BMP2基因表达均显着降低。FD 48 小时后,在所有三个区域都观察到类似的BMP2基因表达模式。两种近视诱导治疗的BMP4BMP7基因表达的变化较小。光学散焦和 FD 引起的BMP变化 鸡 RPE 中的基因表达显示治疗依赖性局部(区域)差异表明局部视网膜和潜在 RPE 区域对眼睛生长调节的性质和贡献存在重要差异。
更新日期:2020-05-26
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