当前位置: X-MOL 学术J. Basic Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of acid and alkali stress on extracellular metabolite profile of Lactobacillus plantarum ATCC 14917
Journal of Basic Microbiology ( IF 3.1 ) Pub Date : 2020-05-26 , DOI: 10.1002/jobm.202000203
Lingyi Wu 1 , Wenwen Wang 1 , Zhen Wu 1 , Daodong Pan 1, 2 , Xiaoqun Zeng 1 , Yuxing Guo 2 , Liwei Lian 3
Affiliation  

As a multifunctional lactic acid bacterium, Lactobacillus plantarum has been proved to survive in the human gastrointestinal tract, and it can also colonize this tract. In this study, the effects of L. plantarum ATCC 14917 metabolic profile caused by initial acid-base (pH 5.5 and 8.5) stress were investigated using 1 H nuclear magnetic resonance spectroscopy and multivariate data analysis. The results showed that the metabolome mainly consisted of 14 metabolites, including the components like amino acids, sugars, organic acids, and alkaloids. According to the nontargeted principal component analysis, there was a decrease in most of the metabolites in the alkali-treated group (mainly change in PC1) except acetate, whereas the production of lactate and glycine was increased in the acid-treated group (mainly change in PC2). Furthermore, the initial alkali stress inhibits the secretion of lactic acid, as a decrease was observed in the activity of lactate dehydrogenase and acetic dehydrogenase of L. plantarum ATCC 14917 in the alkali group. All these findings revealed that alkali stress could limit the acid environment formation of L. plantarum 14917 in the fermentation process; however, low acid pH is more suitable for the growth of L. plantarum.

中文翻译:

酸碱胁迫对植物乳杆菌ATCC 14917胞外代谢产物的影响

作为一种多功能乳酸菌,植物乳杆菌已被证明可以在人体胃肠道中存活,并且也可以定植于该道。在这项研究中,使用 1 H 核磁共振波谱和多变量数据分析研究了由初始酸碱(pH 5.5 和 8.5)胁迫引起的植物乳杆菌 ATCC 14917 代谢特征的影响。结果表明,代谢组主要由14种代谢物组成,包括氨基酸、糖类、有机酸和生物碱等成分。非靶向主成分分析表明,除乙酸外,碱处理组大部分代谢物减少(主要是PC1的变化),而酸处理组乳酸和甘氨酸的产生增加(主要是PC1的变化)在 PC2 中)。此外,初始碱胁迫抑制了乳酸的分泌,因为在碱组中观察到植物乳杆菌 ATCC 14917 的乳酸脱氢酶和乙酸脱氢酶的活性降低。这些结果表明,碱胁迫可以限制植物乳杆菌14917在发酵过程中酸性环境的形成;然而,低酸性pH值更适合植物乳杆菌的生长。
更新日期:2020-05-26
down
wechat
bug