CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome‐editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need for simple approaches with no need for protein engineering. The CRISPR systems usually require a guide RNA (gRNA) moiety to recruit and direct the nuclease complexes. In this respect, the ninhydrin (1,2,3‐indantrione monohydrate) seems to have considerable potential, as yet unexploited, for modifying gRNA. In this study, ninhydrin chemistry is explored for reversible postsynthetic modification of gRNA molecules. It is further shown that ninhydrin chemistry is efficient in modulating two important CRISPR systems. Thus, ninhydrin chemistry exhibits potential applications in future chemical biology studies.

中文翻译：

---

用茚三酮化学处理RNA指导的核酸切割。

CRISPR（成簇的规则间隔的短回文重复序列）系统已被确立为有价值的基因组编辑工具。控制CRISPR系统具有很高的生物学意义，这一领域引起了广泛的关注。非常需要简单的方法而不需要蛋白质工程。CRISPR系统通常需要一个引导RNA（gRNA）部分来募集和指导核酸酶复合物。在这方面，茚三酮（1,2,3-茚满三酮一水合物）似乎具有相当大的潜力，可以用于修饰gRNA。在这项研究中，探索了茚三酮化学法用于gRNA分子的可逆合成后修饰。进一步表明，茚三酮化学在调节两个重要的CRISPR系统方面是有效的。从而，