The Journal of Nutritional Biochemistry ( IF 5.6 ) Pub Date : 2020-05-25 , DOI: 10.1016/j.jnutbio.2020.108429 Anaysa Paola Bolin 1 , Celso Pereira Batista Sousa-Filho 2 , Gustavo Tolentino Nascimento Dos Santos 2 , Letícia Torres Ferreira 2 , Paula Bresciani Martins de Andrade 2 , Ana Carolina Migliorini Figueira 3 , Fernanda Aparecida Heleno Batista 3 , Rosemari Otton 4
The potential contribution of green tea (GT) to the development of thermogenic/beige cells have been scarcely investigated. Here we investigated if the beneficial effects of GT in the induction of thermogenic/beige adipocytes results from an initial cell commitment during adipogenesis. Male C57Bl/6 mice (3 months) were divided into 3 groups: Control (chow diet), Obese (cafeteria diet), and Obese + GT. Mice received GT gavage (500 mg/kg of BW) over 12 weeks (5 days/week), after 4 weeks of diet, totalizing 16 weeks of experimentation. GT treatment increased energy expenditure (EE) in mice fed with cafeteria-diet leading to reduced BW gain, decreased adiposity, reduced inflammation, and improving insulin sensitivity. Those phenotypes were associated with enhanced expression of oxidative, thermogenic and beige genes. GT induced a futile cycle through de novo lipogenesis activating the thermogenic pathway. Induction of beige phenotype occurs autonomously in adipocytes and involves the PPARγ/FGF21/AMPK/UCP1 pathway. Our study identified that metabolic changes caused by GT may involve the temporal expression of PPARγ promoting the induction of thermogenic cells by reprogramming initial steps of adipocyte commitment.
中文翻译:
绿茶多酚诱导的成脂作用将脂肪细胞重塑为生热表型。
几乎没有研究绿茶(GT)对生热/米色细胞发育的潜在贡献。在这里,我们研究了GT在诱导生热/米色脂肪细胞中的有益作用是否源自脂肪形成过程中的初始细胞活动。将雄性C57Bl / 6小鼠(3个月)分为3组:对照组(低脂饮食),肥胖(自助饮食)和肥胖+ GT。饮食4周后,小鼠在12周(5天/周)内接受了GT管饲(500 mg / kg BW),总共进行了16周的实验。GT治疗增加了食堂饮食喂养的小鼠的能量消耗(EE),从而导致BW减少,肥胖,炎症减少和胰岛素敏感性降低。这些表型与氧化,生热和米色基因的表达增强有关。GT通过从头脂肪生成激活产热途径。米色表型的诱导在脂肪细胞中自主发生,涉及PPARγ/ FGF21 / AMPK / UCP1途径。我们的研究发现,由GT引起的代谢变化可能与PPARγ的瞬时表达有关,该过程通过重新编程脂肪细胞定型的初始步骤来促进热原细胞的诱导。