Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) have great potential for regenerative medicine and drug discovery. In this study, we developed a novel protocol to more reproducibly and efficiently induce cardiomyocytes. A large quantity of uniformly sized spheroids were generated from hiPSCs using microfabricated vessels and induced into cardiac differentiation. In the middle of the cardiac differentiation process, spheroids were then dissociated into single cells and reaggregated into smaller spheroids using the microfabricated vessels. This reaggregation process raised WNT5A and WNT11 expression levels and improved the quality of cardiomyocyte population compared to that in a control group in which dissociation and reaggregation were not performed.

从人诱导的多能干细胞（hiPSC）分化出的心肌细胞在再生医学和药物发现方面具有巨大潜力。在这项研究中，我们开发了一种新的协议，以更可重复，更有效地诱导心肌细胞。使用微制造的血管从hiPSC产生了大量均匀大小的球体，并将其诱导为心脏分化。然后，在心脏分化过程的中间，将球体分解成单个细胞，然后使用微细血管将其重新聚集成较小的球体。与未进行解离和重新聚集的对照组相比，该重新聚集过程提高了WNT5A和WNT11的表达水平，并改善了心肌细胞的质量。