当前位置: X-MOL 学术Prog. Biophys. Mol. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Localized reconstruction in Scipion expedites the analysis of symmetry mismatches in cryo-EM data
Progress in Biophysics and Molecular Biology ( IF 3.8 ) Pub Date : 2020-05-26 , DOI: 10.1016/j.pbiomolbio.2020.05.004
Vahid Abrishami 1 , Serban L Ilca 2 , Josue Gomez-Blanco 3 , Ilona Rissanen 1 , José Miguel de la Rosa-Trevín 4 , Vijay S Reddy 5 , José-Maria Carazo 6 , Juha T Huiskonen 7
Affiliation  

Technological advances in transmission electron microscopes and detectors have turned cryogenic electron microscopy (cryo-EM) into an essential tool for structural biology. A commonly used cryo-EM data analysis method, single particle analysis, averages hundreds of thousands of low-dose images of individual macromolecular complexes to determine a density map of the complex. The presence of symmetry in the complex is beneficial since each projection image can be assigned to multiple views of the complex. However, data processing that applies symmetry can average out asymmetric features and consequently data analysis methods are required to resolve asymmetric structural features. Scipion is a cryo-EM image processing framework that integrates functions from different image processing packages as plugins. To extend its functionality for handling symmetry mismatches, we present here a Scipion plugin termed LocalRec implementing the localized reconstruction method. When tested on an adenovirus data set, the plugin enables resolving the symmetry-mismatched trimeric fibre bound to the five-fold vertices of the capsid. Furthermore, it improves the structure determination of the icosahedral capsid by dealing with the defocus gradient across the particle. LocalRec is expected to be widely applicable in a range of cryo-EM investigations of flexible and symmetry mismatched complexes.



中文翻译:

Scipion 中的局部重建加快了冷冻电镜数据中对称性失配的分析

透射电子显微镜和探测器的技术进步使低温电子显微镜 (cryo-EM) 成为结构生物学的重要工具。一种常用的冷冻电镜数据分析方法,即单粒子分析,对单个大分子复合物的数十万个低剂量图像进行平均,以确定复合物的密度图。复合体中对称性的存在是有益的,因为可以将每个投影图像分配给复合体的多个视图。然而,应用对称性的数据处理可以平均化非对称特征,因此需要数据分析方法来解决非对称结构特征。Scipion 是一个冷冻电镜图像处理框架,它以插件的形式集成了来自不同图像处理包的功能。为了扩展其处理对称性不匹配的功能,我们在这里展示了一个名为 LocalRec 的 Scipion 插件,用于实现局部重建方法。在腺病毒数据集上进行测试时,该插件能够解析与衣壳五重顶点结合的对称性不匹配的三聚体纤维。此外,它通过处理粒子上的散焦梯度改进了二十面体衣壳的结构确定。LocalRec 有望广泛应用于灵活和对称错配复合物的一系列冷冻电镜研究。此外,它通过处理粒子上的散焦梯度改进了二十面体衣壳的结构确定。LocalRec 预计将广泛适用于灵活和对称错配复合物的一系列冷冻电镜研究。此外,它通过处理粒子上的散焦梯度改进了二十面体衣壳的结构确定。LocalRec 有望广泛应用于灵活和对称错配复合物的一系列冷冻电镜研究。

更新日期:2020-05-26
down
wechat
bug