The ability of plants to cope with frost events relies on the physiological and molecular responses triggered by cold temperatures. This process, named acclimation, involves reprogramming gene expression in order to adjust metabolism. Planted Eucalyptus species are highly productive but most of them are frost sensitive. However, acclimation process varies among species and environmental conditions, promoting more or less frost damage in young plantations of frost-prone areas. To identify metabolites and proteins responsible for these differences, two acclimation regimes were imposed to seedling of Eucalyptus grandis Hill ex Maiden (Eg), Eucalyptus dunnii Maiden (Ed) and Eucalyptus benthamii Maiden Cambage (Eb), and leaves submitted to biochemical and molecular analyses. Further, seedlings were used for simulated frosts in order to test the acclimation status effect on frost tolerance. Eb showed higher frost tolerance than Ed and Eg under control and acclimation scenarios, possibly due to its higher accumulation of phenolics, anthocyanins and soluble sugars as well as lower levels of photosynthetic pigments and related proteins. Also, a rise in frost tolerance and in osmoprotectants and antioxidants was observed for all the species due to cold acclimation treatment. Interestingly, metabolic profiles differed among species, suggesting different mechanisms to endure frosts and, probably, different requirements for cold acclimation. Shotgun proteomics reinforced differences and commonalities and supported metabolome observations. An in depth understanding of these responses could help to safeguard planted forests productivity through breeding of tolerant genetic material.

植物应对霜冻事件的能力取决于低温触发的生理和分子反应。这个过程称为适应性，涉及重新编程基因表达以调节代谢。种植的桉树种生产力很高，但大多数对霜冻敏感。但是，适应过程随物种和环境条件的不同而不同，从而在易霜冻地区的年轻人工林中或多或少地促进了霜冻的破坏。为了鉴定造成这些差异的代谢物和蛋白质，对两个桉树的幼苗分别施加了两个适应方案：Eucalyptus grandis Hill ex Maiden（Eg），Eucalyptus dunnii Maiden（Ed）和Eucalyptus benthamii处女垃圾（Eb），叶子进行生化和分子分析。此外，将幼苗用于模拟霜冻，以测试驯化状态对霜冻耐受性的影响。Eb的抗冻性比Ed和Eg高在控制和适应环境下，可能是由于其酚类，花色苷和可溶性糖的积累量较高，以及光合色素和相关蛋白的含量较低。此外，由于进行了冷驯化处理，所有物种的抗冻性以及渗透保护剂和抗氧化剂均得到了提高。有趣的是，不同物种之间的代谢特征也不同，这表明承受霜冻的机制不同，并且可能对冷驯化的要求也不同。gun弹枪蛋白质组学加强了差异和共性，并支持代谢组学观察。对这些反应的深入了解可以通过繁殖耐性遗传材料来帮助维护人工林的生产力。