Microbial Pathogenesis ( IF 3.8 ) Pub Date : 2020-05-25 , DOI: 10.1016/j.micpath.2020.104264 Adma Nadja Ferreira de Melo 1 , Geany Targino de Souza Pedrosa 1 , Erika Tayse da Cruz Almeida 2 , Guojie Cao 3 , Dumitru Macarisin 4 , Donald W Schaffner 4 , Evandro L de Souza 2 , Marciane Magnani 1
This study had as aims to evaluate the effects of successive exposures to Mentha piperita L. essential oil (MPEO) on culturability and physiological functions of Salmonella Typhimurium PT4. S. Typhimurium PT4 cells (108 log CFU/mL) were exposed to the same (1.25 μL/mL) or increasing MPEO concentrations (1.25–80 μL/mL) during 252 h. At each 36-h interval, the viable cell counts, and distinct cell functions were assessed using plate counting and flow cytometry, respectively. As the exposure time to the same MPEO concentration increased, the population of S. Typhimurium PT4 cells with damaged, permeabilized and depolarized membrane, and compromised efflux activity decreased. Otherwise, S. Typhimurium PT4 cells with damaged membrane physiological functions increased over the exposure to increasing concentrations of MPEO. Genomic analyses showed that the strain carries 17 genes associated with stress responses and the persistence of the tested strain among sources associated with poultry spanning more than 16 years and its virulence for humans. Therefore, successive exposure to a sublethal concentration of MPEO induced S. Typhimurium PT4 cells capable of maintaining the membrane integrity and its functions despite their non-culturable state.
中文翻译:
在持续流行的鼠伤寒沙门氏菌PT4中,连续接触薄荷油精油会影响其可培养性并诱导膜修复。
这项研究的目的是评估连续接触薄荷薄荷精油(MPEO)对鼠伤寒沙门氏菌PT4的可培养性和生理功能的影响。S.鼠伤寒沙门氏菌PT4细胞(10 8日志CFU / mL)的暴露于相同的(1.25μL/ mL)或252小时内增加MPEO浓度(1.25-80μL/ mL)中。在每个36小时间隔,分别使用平板计数和流式细胞术评估存活细胞计数和不同的细胞功能。作为曝光时间相同MPEO浓度的增加,人口S.鼠伤寒沙门氏菌PT4细胞与受损,透化和去极化膜,和受损流出活性降低。否则,S。膜生理功能受损的鼠伤寒PT4细胞随着MPEO浓度的增加而增加。基因组分析表明,该菌株携带17个与应激反应相关的基因,并且在超过16年的家禽相关来源及其对人类的毒力中存在着被测菌株的持久性。因此,连续暴露于MPEO的亚致死浓度诱导的小号。鼠伤寒PT4细胞尽管不能培养也能维持膜的完整性及其功能。