Gene ( IF 3.5 ) Pub Date : 2020-05-26 , DOI: 10.1016/j.gene.2020.144809 Shuqing Yang 1 , Ningning Yan 2 , Klaas Bouwmeester 3 , Ren Na 4 , Zhiwei Zhang 2 , Jun Zhao 2
Small GTPases function as molecular switches to active or inactive signaling cascades via binding or hydrolyzing GTP. A type of plant specific small GTPases, the ROPs are known to be involved in plant growth, development and immunity. We determined whether ROPs are conserved in Solanaceous species and whether they are involved in plant growth, development and resistance against Phytophthora capsisi. In genome-wide screening, a total of 66 ROPs in six Solanaceous species (SolROPs) were identified, including 16 ROPs in Solanum tuberosum L. (potato), 9 in Solanum lycopersicum L. (tomato), 5 in Solanum melongena L. (eggplant), 9 in Capsicum annuum L. (pepper), 13 in Nicotiana benthamiana Domin and 14 in Nicotiana tabacum L. (tobacco). Phylogenetic analysis revealed that 11 AtROPs and 66 SolROPs fall into five distinct clades (I-V) and hence a novel and systematic gene nomenclature was proposed. In addition, a comprehensive expression analysis was performed by making use of an online database. This revealed that ROP genes are differentially expressed during plant growth and development. Moreover, gene expression of SlROP-II.1 in S. lycopersicum could be significantly induced by P. capsici. Subsequently, SlROP-II.1 and its homologues in N. benthamiana and C. annuum (NbROP-II.1 and CaROP-II.1) were selected for functional analysis using virus-induced gene silencing. Infection assays with P. capsici on silenced plants revealed that SlROP-II.1, NbROP-II.1 and CaROP-II.1 play a role in P. capsici resistance, suggesting conserved function of ROP-II clade across different Solanaceous species. In addition, NbROP-II.1 is also involved in regulating plant growth and development. This study signified the diversity of Solanaceous ROPs and their potential roles in plant growth, development and immunity.
中文翻译:
小G蛋白ROP的全基因组鉴定及其在茄科中的潜在作用。
小型GTPases通过结合或水解GTP充当分子切换至有活性或无活性信号传导级联的分子。ROP是一种植物特有的小GTP酶,已知与植物的生长,发育和免疫有关。我们确定ROPs在茄科物种中是否保守,以及它们是否参与植物的生长,发育以及对辣椒疫霉菌的抗性。在全基因组筛选,在六个共66个像素管线茄科物种(SolROPs)被确定,其中包括在16点的ROP马铃薯L.(马铃薯),9在番茄L.(番茄),5在茄子L.(茄子),9 in Capsicum annuumL.(pepper),本特烟草(Nicotiana benthamiana Domin )的13个和烟草(Nicotiana tabacum L.)的14个。系统发育分析表明,有11个AtROP和66个SolROP落入五个不同的进化枝(IV)中,因此提出了一种新颖而系统的基因命名法。另外,利用在线数据库进行了全面的表达分析。这表明ROP基因在植物生长和发育过程中差异表达。此外,基因表达SlROP-II.1在番茄可以显著诱导辣椒疫霉。随后,SlROP-II.1及其在本氏猪笼草和C. annuum中的同源物(NbROP-II.1和CaROP-II.1)被选择用于使用病毒诱导的基因沉默的功能分析。与感染检测辣椒疫霉上沉默的植物的发现,SlROP-二.1,NbROP-.1和CaROP-II.1中发挥作用辣椒疫霉性,这表明在不同的ROP-II进化保守功能茄果类物种。此外,NbROP-II.1还参与调节植物的生长和发育。这项研究表明茄科ROPs的多样性及其在植物生长,发育和免疫中的潜在作用。