In this study, we report the antioxidant and antitoxic potential of chemically synthesized 4-oxo-2-phenyl-4H-chromene-7,8-diyl bis((1-amino-2-hydroxypropyl)carbamate) (DHF-BAHPC) compound using in vitro and in vivo assays. The DHF-BAHPC was synthesized by linking 7,8-Dihydroxyflavone (DHF) with two molecules of Fmoc-threonine and characterized by Ultraviolet-visible spectroscopy (UV–vis), Fourier-transform infrared spectroscopy (FT-IR), ¹H NMR, ¹³C NMR and Mass spectrometry (MS). In vitro, antioxidant assay results revealed that DHF-BAHPC has a dose-dependent radical scavenging potential towards DPPH, ABTS, FRAP and H₂O₂ radicals with an IC₅₀ range of 15.45, 66.27, 25.71, 4.375 μg/mL, respectively. Furthermore DHF-BAHPC treatment significantly altered cadmium (Cd) intoxicated zebrafish embryos by rescuing the developmental changes associated with severe histological and reduced the level of defensive antioxidant activities (SOD, CAT, GPx and GST). The overall results of the present study represented that DHF-BAHPC may be used as a potential drug in redox-based therapeutics.

在这项研究中，我们报告了化学合成的4-氧代-2-苯基-4 H-苯甲基-7,8-二基双（（1-氨基-2-羟丙基）氨基甲酸酯）（DHF-BAHPC）的抗氧化剂和抗毒潜力使用体外和体内试验测定化合物。DHF-BAHPC是通过将7,8-二羟基黄酮（DHF）与两个Fmoc-苏氨酸分子连接而合成的，并通过紫外-可见光谱（UV-vis），傅立叶变换红外光谱（FT-IR），¹ H NMR进行表征，¹³ C NMR和质谱（MS）。在体外，抗氧化剂检测结果表明DHF-BAHPC具有剂量依赖性的清除DPPH，ABTS，FRAP和H ₂ O ₂自由基的能力，IC _50为范围分别为15.45、66.27、25.71和4.375μg/ mL。此外，DHF-BAHPC处理通过挽救与严重组织学相关的发育变化并降低了防御性抗氧化活性（SOD，CAT，GPx和GST）的水平，大大改变了镉（Cd）中毒的斑马鱼胚胎。本研究的总体结果表明，DHF-BAHPC可用作基于氧化还原的疗法中的潜在药物。