We studied the causal variation, prognosis and risk factors of parvo viral enteritis using both molecular and statistical analysis. A multivariate factor analysis was performed to see the effect of the breed, age, vaccination status and the leukocyte counts on disease severity and survival. Twenty PCR amplicons (partial VP2 gene) from vaccinated dogs were sequenced to identify the variants. A total of 109 samples were positive for parvo viral DNA, 71 of which were from dogs who have received at least one dose of CPV-2 vaccine. Over 90% were leukopenic on 2^nd and 3^rd day of diarrhoea. Age < 6 months was negatively correlated with leukocyte count and the leukocyte count was negatively correlated with survival. Dobermans and Labradors showed significant negative correlation with survival. All variants of parvovirus affected dogs with the 2c being the predominant (2c = 12, 2a = 7, 2b = 1). All 20 strains harboured three additional amino acid substitutions (i.e. Phe267Tyr, Ser297Ala and Tyr324Ile) and warrant further studies on potential changes of the antigenicity of the virus.

我们使用分子和统计分析研究了细小病毒性肠炎的病因变异，预后和危险因素。进行了多因素因子分析，以查看品种，年龄，疫苗接种状况以及白细胞计数对疾病严重性和存活率的影响。对来自疫苗接种的狗的20个PCR扩增子（部分VP2基因）进行测序以鉴定变体。总共109份小病毒DNA阳性，其中71份来自至少接受过一剂CPV-2疫苗的狗。超过90％的患者在^第二和^第三次出现白细胞减少腹泻的日子。年龄<6个月与白细胞计数呈负相关，白细胞计数与存活率呈负相关。杜宾犬和拉布拉多犬与存活率呈显着负相关。细小病毒感染的所有变体均以2c为主导（2c = 12、2a = 7、2b = 1）。所有这20个菌株都具有三个附加的氨基酸取代（即Phe267Tyr，Ser297Ala和Tyr324Ile），因此有必要进一步研究病毒抗原性的潜在变化。