Abstract 2,5-Furandicarboxylic acid (FDCA) is one of the most important platform chemicals especially as a promising alternative to terephthalic acid. In this study, a new and prospective tandem whole-cell cascade composed of recombinant Escherichia coli and wild Pseudomonas putida KT2440 was constructed for FDCA production from 5-hydroxymethylfurfural (HMF) based on their respective alcohol and aldehyde oxidation activities. In particular, P. putida proved superior biocatalytic ability to furanic aldehydes. In the stepwise reaction, HMF (up to 100 mM) was fully oxidized to FDCA, with bioconversion rate much faster than other reported biocatalysts either as single enzyme or as combinations. Besides, this study is the first time to construct a whole-cell cascade system achieving FDCA production with quantitative yield.

中文翻译：

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通过将大肠杆菌中的异源途径与恶臭假单胞菌中的天然途径偶联来改进 2,5-呋喃二甲酸的生物合成

摘要 2,5-呋喃二甲酸（FDCA）是最重要的平台化学品之一，特别是作为对苯二甲酸的有前途的替代品。在这项研究中，构建了一个由重组大肠杆菌和野生恶臭假单胞菌 KT2440 组成的新的、前瞻性的串联全细胞级联，用于基于它们各自的醇和醛氧化活性从 5-羟甲基糠醛 (HMF) 生产 FDCA。特别是，恶臭假单胞菌证明了对呋喃醛的优异生物催化能力。在逐步反应中，HMF（高达 100 mM）被完全氧化为 FDCA，其生物转化率比其他报道的生物催化剂作为单一酶或组合快得多。此外，本研究首次构建了全细胞级联系统，实现了定量产率的FDCA生产。