Ringer's lactate solution irradiated by non-thermal plasma, comprised of radicals, electrons, and ions, is defined as plasma-activated lactate (PAL). PAL exhibited antitumor effects in glioblastoma U251SP cells, which we termed PAL-specific regulated cell death. In contrast to the oxidative stress condition typical of cells incubated in plasma-activated medium (PAM), U251SP cells treated with Ringer's lactate solution or PAL exhibited changes in intracellular metabolites that were reductive in the redox state, as measured by the ratio of oxidative/reductive glutathione concentrations. In the metabolomic profiles of PAL-treated cells, the generation of acetyl-CoA increased for lipid metabolism from alanine and asparagine. PAL thus induces regulated death of U251SP glioblastoma cells in more innate microenvironments than PAM.

由自由基，电子和离子组成的非热等离子体辐射的林格氏乳酸溶液定义为等离子体活化的乳酸（PAL）。PAL在胶质母细胞瘤U251SP细胞中表现出抗肿瘤作用，我们称其为PAL特异性调控的细胞死亡。与在血浆激活培养基（PAM）中孵育的细胞典型的氧化应激条件相反，用林格氏乳酸盐溶液或PAL处理的U251SP细胞在氧化还原状态下还原的细胞内代谢产物表现出变化，如氧化/氧化比还原型谷胱甘肽浓度。在PAL处理的细胞的代谢组学谱中，乙酰丙氨酸辅酶A的生成增加了丙氨酸和天冬酰胺的脂质代谢。因此，与PAM相比，PAL在更先天的微环境中诱导U251SP胶质母细胞瘤细胞的调控死亡。