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Characteristics and Functions of PePIF3, a Gene Related to Circadian Rhythm in “Nanlin 895” Poplar
Plant Molecular Biology Reporter ( IF 2.1 ) Pub Date : 2020-05-26 , DOI: 10.1007/s11105-020-01215-0 Xiaolong Wu , Ling Liu , Qi Xu , Hui Wei , Xiaoli Wang , Weibo Sun , Qiang Zhuge
Plant Molecular Biology Reporter ( IF 2.1 ) Pub Date : 2020-05-26 , DOI: 10.1007/s11105-020-01215-0 Xiaolong Wu , Ling Liu , Qi Xu , Hui Wei , Xiaoli Wang , Weibo Sun , Qiang Zhuge
Plants coordinate metabolism and other physiological processes via circadian rhythms to cope with the diurnally changing environment. Light is the most important factor influencing circadian clock gene expression. Multi-level interactions occur between the plant circadian clock and the environment, and many circadian clock-related genes exhibit a positive effect in environmental changes on different time scales. Phytochrome-interacting factors (PIFs) are transcription factors with a basic helix-loop-helix (bHLH) domain; they interact with phytochromes (Phys) and play an important role in the early steps of light signal transduction. PIF3 is a member found using a yeast two-hybrid screen for phytochrome B-interacting proteins and functions as a positive regulator mediating tolerance to dehydration and salt stress. In this study, diurnal changes in the “Nanlin 895” poplar leaves transcriptome in natural light were analyzed using RNA sequencing. The Nanlin 895 poplar transcriptome was found to have > 92% similarity with that of Populus trichocarpa. A total of 11,266 differentially expressed genes were identified from 34,869 genes. Among these, we determined via the k-means clustering method that 1067 genes had at least one expression peak at some time during the day. Principal components and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated that light was the key factor altering the transcriptome. The full-length coding sequences of PePIF3a and PePIF3b, which encode PIF3 proteins in Nanlin 895 poplar, were cloned; sequence analysis indicated that PePIF3a and PePIF3b were unstable proteins with conserved bHLH, active phytochrome B, and active phytochrome A domains. PePIF3a functioned as positive regulator in drought and salt stress responses. These findings provide a basis for tree breeding to enhance their adaptability to adverse environments.
更新日期：2020-05-26
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