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Age-Related Effects of Orthovanadate Nanoparticles Involve Activation of GSH-Dependent Antioxidant System in Liver Mitochondria.
Biological Trace Element Research ( IF 3.9 ) Pub Date : 2020-05-24 , DOI: 10.1007/s12011-020-02196-7
Yuri V Nikitchenko 1 , Vladimir K Klochkov 2 , Nataliya S Kavok 2 , Nina A Karpenko 2 , Svetlana L Yefimova 2 , Irina V Nikitchenko 1 , Anatoly I Bozhkov 1
Affiliation  

Vanadium is an important ultra-trace element nowadays attracting attention with particular emphasis on medical application. But the therapeutic application of vanadium-based drugs is still questionable and restricted due to some toxic side effects. It was found that unique redox properties of vanadium in nanoform provided antioxidant activity and prevented oxidative disturbance in cells in vitro. Though, on the organism level, ambiguous effects of vanadium-based nanoparticles were observed. In this study, the age-related features of prooxidant/antioxidant balance in blood serum and liver mitochondrial and postmitochondrial fractions of 3 and 18-month-old Wistar male rats treated with orthovanadate nanoparticles (GdVO4/Eu3+, 8 × 25 nm) within 2 months have been investigated. Prooxidant potential-related indexes were the content of lipid hydroperoxides as well as aconitase activity. Activity of glutathione peroxidase, glutathione-S-transferase, glutaredoxin, glutathione reductase, glucose-6-phosphate dehydrogenase, and NADPH-dependent isocitrate dehydrogenase designated the tissue antioxidant potential. Based on the obtained values, the integral index of the prooxidant/antioxidant balance-the reliability coefficient (Kr) has been calculated. The data show that due to activation some chain links of GSH-dependent antioxidant system, GdVO4/Eu3+ nanoparticles increase the reliability of the prooxidant-antioxidant balance in tissues and especially in the liver mitochondria of old animals (Kr in mitochondria of young rats was 2.94, and in mitochondria of old ones-9.83 conventional units). Detected in vitro glutathione peroxidase-like activity of the GdVO4/Eu3+ nanoparticles is supposed to be among factors increasing the reliability of the system. So, for the first time, the beneficial effect of the long-term orthovanadate nanoparticle consumption in old males has been discovered.

中文翻译:

原钒酸盐纳米颗粒的年龄相关效应涉及肝线粒体中 GSH 依赖性抗氧化系统的激活。

钒是一种重要的超微量元素,目前备受关注,尤其是在医学上的应用。但由于一些毒副作用,钒基药物的治疗应用仍然存在问题并受到限制。发现纳米形式的钒独特的氧化还原特性提供了抗氧化活性并防止了体外细胞的氧化干扰。但是,在生物体水平上,观察到了钒基纳米粒子的模糊影响。在这项研究中,用原钒酸盐纳米粒子(GdVO4/Eu3+,8 × 25 nm)处理的 3 个月和 18 个月大的 Wistar 雄性大鼠的血清和肝脏线粒体和线粒体后组分中促氧化/抗氧化平衡的年龄相关特征在 2月已被调查。促氧化电位相关指标为脂质氢过氧化物含量和乌头酸酶活性。谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、谷氧还蛋白、谷胱甘肽还原酶、6-磷酸葡萄糖脱氢酶和 NADPH 依赖性异柠檬酸脱氢酶的活性指定了组织抗氧化潜力。基于获得的值,计算了促氧化/抗氧化平衡的积分指数-可靠性系数(Kr)。数据表明,由于激活了 GSH 依赖的抗氧化系统的一些链节,GdVO4/Eu3+ 纳米粒子增加了组织中促氧化 - 抗氧化平衡的可靠性,尤其是在老年动物的肝线粒体中(幼鼠线粒体中的 Kr 为 2.94 ,以及旧的线粒体 - 9.83 常规单位)。检测到的 GdVO4/Eu3+ 纳米颗粒的体外谷胱甘肽过氧化物酶样活性被认为是提高系统可靠性的因素之一。因此,首次发现了老年男性长期服用原钒酸盐纳米颗粒的有益效果。
更新日期:2020-05-24
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