The need for alternative approaches for identifying pathogens has led researchers to focus on nanobiotechnology. In this study, zinc oxide nanoparticles (ZnO NPs) and multi-wall carbon nanotubes (MWCNTs) were used as marker molecules. After measuring the best concentration of these nanomaterials to inhibit the lactase activity of the beta-galactosidase enzymes by binding to them, different concentrations of Escherichia coli were added to the medium and their detection ability was finally compared with each other. Due to small size and high reactivity, these compounds are able to detect very low amount of bacteria in the ambient. In fact, the bacteria are attached to the nanoparticles and detach them from the enzyme and lead to substrate decomposition by the enzyme. MWCNTs exhibited better performance than ZnO NPs in detection of bacteria at very low concentration of 101 CFU/ml in 15 min. As a result, they are very appropriate to be utilized especially in the food industry.

中文翻译：

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基于 ZnO NPs 和 MWCNTs 的 β-半乳糖苷酶生物传感器快速检测大肠杆菌：比较研究

对识别病原体的替代方法的需求促使研究人员专注于纳米生物技术。在这项研究中，氧化锌纳米粒子 (ZnO NPs) 和多壁碳纳米管 (MWCNTs) 被用作标记分子。在测量这些纳米材料通过与它们结合来抑制β-半乳糖苷酶的乳糖酶活性的最佳浓度后，将不同浓度的大肠杆菌加入培养基中，最后比较它们的检测能力。由于体积小且反应性高，这些化合物能够检测到环境中极少量的细菌。事实上，细菌附着在纳米颗粒上并将它们与酶分离，并导致酶分解底物。MWCNTs 在 15 分钟内以 101 CFU/ml 的极低浓度检测细菌时表现出比 ZnO NPs 更好的性能。因此，它们非常适合用于食品行业。