Determination of a stem cell source with sufficient myogenic differentiation capacity that can be easily obtained in large quantities is of great importance in skeletal muscle regeneration therapies. Adipose-derived stem cells (ASCs) are readily available, can be isolated from fat tissue with high yield and possess myogenic differentiation capacity. Consequently, ASCs have high applicability in muscle regenerative therapies. However, a key challenge is their low differentiation efficiency. In this study, we have explored the potential of mimicking the natural microenvironment of the skeletal muscle tissue to enhance ASC myogenesis by inducing 3D cellular alignment and using dynamic biomimetic culture. ASCs were entrapped and 3D aligned in parallel within fibrin-based microfibers and subjected to uniaxial cyclic stretch. 3D cell alignment was shown to be necessary for achieving and maintaining the stiffness of the construct mimicking the natural tissue (12 ± 1 kPa), where acellul...

中文翻译：

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3D 细胞排列和仿生机械刺激增强人类脂肪来源的干细胞肌生成。

确定具有足够的生肌分化能力且易于大量获得的干细胞来源在骨骼肌再生治疗中非常重要。脂肪来源的干细胞 (ASC) 很容易获得，可以从脂肪组织中以高产量分离，并具有生肌分化能力。因此，ASCs 在肌肉再生治疗中具有很高的适用性。然而，一个关键的挑战是它们的低分化效率。在这项研究中，我们探索了通过诱导 3D 细胞排列和使用动态仿生培养来模拟骨骼肌组织的自然微环境以增强 ASC 肌生成的潜力。ASCs 被截留并平行排列在基于纤维蛋白的微纤维内并进行单轴循环拉伸。