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ZDHHC5 targets Protocadherin 7 to the cell surface by a palmitoylation-dependent mechanism to promote successful cytokinesis
bioRxiv - Cell Biology Pub Date : 2022-05-20 , DOI: 10.1101/2020.05.24.111831
Nazlı Ezgi Özkan Küçük , Berfu Nur Yiğit , Beste Senem Değirmenci , Mohammad Haroon Qureshi , Altuğ Kamacıoğlu , Nima Bavili , Alper Kiraz , Nurhan Özlü

Cell division requires dramatic reorganization of the cell cortex that is primarily driven by the actomyosin network. We previously reported that Protocadherin 7 (PCDH7) enriches at the cell surface during mitosis which is required for building up the full mitotic rounding pressure. Here we showed that PCDH7 gets palmitoylated and interacts with the palmitoyltransferase, ZDHHC5. Both PCDH7 and ZDHHC5 co-localize at the mitotic cell surface and get enriched at the cleavage furrow during cytokinesis. PCDH7's localization is ZDHHC5 dependent. Loss of expression of ZDHHC5 or PCDH7 leads to defects in cleavage furrow ingression and cytokinesis. At the mitotic cell surface and cleavage furrow, PCDH7 interacts with Myosin Phosphatase Target Subunit1 (MYPT1). We propose that this interaction tunes phospho-myosin levels at the cleavage furrow for regulating the myosin activity during cytokinesis. This work uncovers a palmitoylation-dependent translocation for PCDH7 which regulates myosin activity at the cortex and cleavage furrow for a successful division in human cells.

中文翻译:

ZDHHC5 通过棕榈酰化依赖性机制将原钙粘蛋白 7 靶向细胞表面以促进成功的胞质分裂

细胞分裂需要对主要由肌动球蛋白网络驱动的细胞皮层进行剧烈重组。我们之前报道过,在有丝分裂过程中,原钙粘蛋白 7 (PCDH7) 在细胞表面富集,这是建立完整的有丝分裂舍入压力所必需的。在这里,我们展示了 PCDH7 被棕榈酰化并与棕榈酰转移酶 ZDHHC5 相互作用。PCDH7 和 ZDHHC5 都在有丝分裂细胞表面共定位,并在胞质分裂期间在卵裂沟处富集。PCDH7 的定位依赖于 ZDHHC5。ZDHHC5 或 PCDH7 的表达缺失导致卵裂沟进入和胞质分裂的缺陷。在有丝分裂细胞表面和分裂沟处,PCDH7 与肌球蛋白磷酸酶靶亚基 1 (MYPT1) 相互作用。我们建议这种相互作用调节卵裂沟处的磷酸肌球蛋白水平,以调节胞质分裂过程中的肌球蛋白活性。这项工作揭示了 PCDH7 的棕榈酰化依赖性易位,该易位可调节皮层肌球蛋白活性和卵裂沟,从而在人类细胞中成功分裂。
更新日期:2022-05-23
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