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Potential of novel food-borne Lactobacillus isolates against the honeybee pathogen Paenibacillus larvae
Biocontrol Science and Technology ( IF 1.4 ) Pub Date : 2020-05-25 , DOI: 10.1080/09583157.2020.1769556
Anna Marta Lazzeri 1 , Nicoletta P. Mangia 1 , Maria Elena Mura 1 , Ignazio Floris 1 , Alberto Satta 1 , Luca Ruiu 1
Affiliation  

ABSTRACT An in vitro study was conducted to screen for the inhibitory potential of lactic acid bacteria (LAB), isolated from fermented food matrices, against the honeybee American Foulbrood agent Paenibacillus larvae. Various strains of Lactobacillus plantarum showed significant antagonism associated with their cell-free culture supernatant (CFS). The characterisation of this inhibition was based on assays and analyses involving different fraction and extracts. A significant effect was associated with media acidification resulting from LAB growth. However, this antimicrobial activity was found to be heat-stable and specific experiments suggested the implication of small antimicrobial peptides and/or antibiotics. Accordingly, a possible role of plantaricins released by L. plantarum in the culture supernatant was confirmed by the expression of different plantaricin-related genes. The results obtained in this study support further investigations on LAB strains as biological control agents for application in the apiary to preserve and improve honeybee health.

中文翻译:

新型食源性乳杆菌分离株对抗蜜蜂病原体类芽孢杆菌幼虫的潜力

摘要 进行了一项体外研究,以筛选从发酵食品基质中分离的乳酸菌 (LAB) 对蜜蜂美国 Foulbrood 代理类芽孢杆菌幼虫的抑制潜力。各种植物乳杆菌菌株显示出与其无细胞培养上清液 (CFS) 相关的显着拮抗作用。这种抑制的表征基于涉及不同级分和提取物的测定和分析。显着的影响与 LAB 生长导致的培养基酸化有关。然而,发现这种抗微生物活性是热稳定的,并且具体实验表明小抗微生物肽和/或抗生素的含义。因此,L. 释放的植物素的可能作用。通过不同植物素相关基因的表达证实了培养上清液中的植物乳杆菌。本研究中获得的结果支持进一步研究 LAB 菌株作为生物控制剂在养蜂场中应用以保护和改善蜜蜂健康。
更新日期:2020-05-25
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