Abstract

Microfluidic capillary electrophoresis (MCE) is the novel technique resulted from the CE mininaturization as planar separation and analysis device. This review presents and discusses various application fields of this advanced technology published in the period 2017 till mid-2019 in eight different sections including clinical, biological, single cell analysis, environmental, pharmaceuticals, food analysis, forensic and ion analysis. The need for miniaturization of CE and the consequence advantages achieved are also discussed including high-throughput, miniaturized detection, effective separation, portability and the need for micro- or even nano-volume of samples. Comprehensive tables for the MCE applications in the different studied fields are provided. Also, figure comparing the number of the published papers applying MCE in the eight discussed fields within the studied period is included. The future investigation should put into consideration the possibility of replacing conventional CE with the MCE after proper validation. Suitable validation parameters with their suitable accepted ranges should be tailored for analysis methods utilizing such unique technique (MCE).

摘要

微流体毛细管电泳 (MCE) 是 CE 小型化作为平面分离和分析设备而产生的新技术。本综述介绍并讨论了 2017 年至 2019 年中期发表的这项先进技术的各个应用领域，分为临床、生物学、单细胞分析、环境、药物、食品分析、法医和离子分析等八个不同部分。还讨论了对 CE 小型化的需求以及所实现的后果优势，包括高通量、小型化检测、有效分离、便携性以及对微量甚至纳米量样品的需求。提供了不同研究领域中 MCE 应用程序的综合表格。还，包括比较研究期间在八个讨论领域中应用 MCE 的已发表论文数量的图表。未来的调查应考虑在适当验证后用 MCE 替换传统 CE 的可能性。应为使用这种独特技术 (MCE) 的分析方法量身定制合适的验证参数及其合适的可接受范围。