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Preliminary Trichinella spiralis Infection Ameliorates Subsequent RSV Infection-Induced Inflammatory Response.
Cells ( IF 6 ) Pub Date : 2020-05-25 , DOI: 10.3390/cells9051314
Ki-Back Chu 1 , Hae-Ahm Lee 2 , Hae-Ji Kang 1 , Eun-Kyung Moon 3 , Fu-Shi Quan 2, 3
Affiliation  

Respiratory syncytial virus (RSV) infection affects the lives of neonates throughout the globe, causing a high rate of mortality upon hospital admission. Yet, therapeutic options to deal with this pulmonary pathogen are currently limited. Helminth therapy has been well received for its immunomodulatory role in hosts, which are crucial for mitigating a multitude of diseases. Therefore, in this study, we used the helminth Trichinella spiralis and assessed its capabilities for modulating RSV infection as well as the inflammatory response induced by it in mice. Our results revealed that RSV-specific antibody responses were enhanced by pre-existing T. spiralis infection, which also limited pulmonary viral replication. Diminished lung inflammation, indicated by reduced pro-inflammatory cytokines and inflammatory cell influx was confirmed, as well as through histopathological assessment. We observed that inflammation-associated nuclear factor kappa-light-chain enhancement of activated B cells (NF-κB) and its phosphorylated forms were down-regulated, whereas antioxidant-associated nuclear factor erythroid 2-related factor 2 (Nrf2) protein expression was upregulated in mice co-infected with T. spiralis and RSV. Upregulated Nrf2 expression contributed to increased antioxidant enzyme expression, particularly NQO1 which relieved the host of oxidative stress-induced pulmonary inflammation caused by RSV infection. These findings indicate that T. spiralis can mitigate RSV-induced inflammation by upregulating the expression of antioxidant enzymes.

中文翻译:

初步旋毛虫感染可缓解随后的RSV感染引起的炎症反应。

呼吸道合胞病毒(RSV)感染会影响全球新生儿的生命,导致入院时死亡率很高。然而,目前治疗这种肺病原体的治疗选择有限。蠕虫疗法因其在宿主中的免疫调节作用而广受好评,这对于减轻多种疾病至关重要。因此,在这项研究中,我们使用了蠕虫旋毛虫,并评估了其调节RSV感染的能力以及由其诱发的小鼠炎症反应。我们的研究结果表明,预先存在的螺旋螺旋体可增强RSV特异性抗体反应感染,这也限制了肺病毒的复制。通过减少促炎细胞因子和炎性细胞流入,以及通过组织病理学评估,证实了肺炎症减轻。我们观察到,炎症相关核因子κ轻链增强的活化B细胞(NF-κB)及其磷酸化形式被下调,而抗氧化剂相关核因子红系2相关因子2(Nrf2)的蛋白表达在与螺旋螺旋体和RSV共同感染的小鼠中表达上调。Nrf2表达上调导致抗氧化酶表达增加,特别是NQO1减轻了由RSV感染引起的氧化应激诱导的肺部炎症的宿主。这些发现表明螺旋螺旋体可以通过上调抗氧化酶的表达来减轻RSV诱导的炎症。
更新日期:2020-05-25
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