Propionibacterium acnes plays an important role in the pathophysiology of acne vulgaris, the most common chronic inflammatory skin disease of the pilosebaceous unit. This study was conducted to investigate whether the entomopathogenic fungus Polycephalomyces phaothaiensis components have antibacterial and anti-inflammatory effects against P. acnes that may serve for acne treatment. A chemical study by spectroscopic analysis resulted in the identification of seven known compounds. The anti-P. acnes potency of extracts and test compounds was determined by both agar diffusion and broth dilution methods. The ethyl acetate extract from culture broth along with cordytropolone (1) and stipitalide (2) exhibited strong anti- P. acnes activity while (+)-piliformic acid (3) showed mild inhibitory activity. The anti-inflammatory effect of ethyl acetate extract and 1–3 was then examined by the quantification of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α on heat-killed P. acnes induced cytokine production by THP-1 cells. The result demonstrated that the extract and its constituents (1–3) showed a potent significant effect by inhibiting the P. acnes-induced pro-inflammatory cytokines production in THP-1. Our results suggest for the first time that P. phaothaiensis and its constituents (1 and 2) hold therapeutic value for further studies as a new alternative treatment for acne.

中文翻译：

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新型昆虫真菌phaothaiensis phaothaiensis提取物及其成分对痤疮丙酸杆菌的体外抗菌和抗炎作用。

痤疮丙酸杆菌在寻常痤疮的病理生理中起重要作用，寻常痤疮是痤疮的最常见的慢性炎症性皮肤病。进行这项研究的目的是调查昆虫病原性真菌phaothaiensis的多头菌属成分是否对可能用于治疗痤疮的痤疮丙酸杆菌具有抗菌和消炎作用。通过光谱分析进行化学研究，鉴定出了七个已知化合物。提取物和测试化合物的抗痤疮丙酸杆菌效力通过琼脂扩散法和肉汤稀释法确定。培养液中的乙酸乙酯提取物与虫草酮（1）和乙ital（2）。）表现出很强的抗痤疮丙酸杆菌活性，而（+）-聚甲酸（3）表现出温和的抑制活性。乙酸乙酯提取物和所述的消炎作用1 - 3然后通过促炎性细胞因子IL-1β的定量，IL-6和TNF-α对热灭活检测痤疮丙酸杆菌由THP-1诱导的细胞因子的产生细胞。结果表明，所述提取物和其组分（1 - 3）显示出通过抑制一种有效的显著效果痤疮丙酸杆菌诱导的促炎性细胞因子生产在THP-1。我们的研究结果首次表明，P。phaothaiensis及其成分（1和2）具有治疗价值，作为痤疮的新替代疗法有待进一步研究。