The aim of this work is to establish the most suitable proteolysis conditions to obtain bovine casein hydrolysates containing peptides with antioxidant and antihypertensive capacity. To this end, the proteolytic activity of Cynara scolymus L. flower extracts was characterized on whole bovine casein, evaluating the effect of several factors (pH, temperature, substrate concentration, enzyme concentration, and hydrolysis time). The optimal conditions to carry out the hydrolysis with the C. scolymus L. extract were as follows: pH 6.2, 50 °C, and 0.023 mg·mL⁻¹ of extract-protein concentration. A Michaelis constant (K_m) value of 5.66 mg·mL⁻¹ and a maximum rate of reaction (V_max) of 8.47 mUAbs∙min⁻¹ were observed. The optimal hydrolysis time was 17 h. The casein hydrolysates obtained with these conditions contained peptides with antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity: 30.89%; Trolox equivalent antioxidant capacity (TEAC) against 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free radical (ABTS^●+): 4.43 mM Trolox equivalent·mg⁻¹ peptide) and antihypertensive activity, showing 55.05% angiotensin-converting enzyme-I inhibition in vitro.

中文翻译：

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朝鲜蓟（Cynara scolymus L.）花提取物在牛酪蛋白上获得生物活性肽的蛋白水解活性的表征。

这项工作的目的是建立最合适的蛋白水解条件，以获得含有具有抗氧化和抗高血压能力的肽的牛酪蛋白水解产物。为此，表征了Cynara scolymus L.花提取物对全牛酪蛋白的蛋白水解活性，评估了几个因素（pH，温度，底物浓度，酶浓度和水解时间）的影响。的最佳条件，进行与水解朝鲜蓟L.提取物如下：pH 6.2的，50℃，和0.023毫克·毫升^{- 1}提取物的蛋白浓度。甲米氏常数（K_米的5.66毫克·毫升）值^{- 1}和反应的最大速率（V_最大值）为8.47 mUAbs∙min ^{− 1}。最佳水解时间为17 h。在这些条件下获得的酪蛋白水解物含有具有抗氧化活性的肽（1,1-二苯基-2-吡啶并肼基（DPPH）自由基清除能力：30.89％;对2,2'-叠氮基双（3）的Trolox等效抗氧化能力（TEAC） -乙基苯并噻唑啉-6-磺酸）自由基（ABTS ^●+）：4.43 mM Trolox当量·mg ^{- 1}肽）和降压活性，在体外显示55.05％的血管紧张素转化酶I抑制作用。