Porcine myometrium possesses steroidogenic activity but its regulation is not well understood. It was hypothesized that the regulators of myometrial steroidogenesis are insulin-like growth factor 1 (IGF-1) and epidermal growth factor (EGF), which were found to modulate the steroidogenic activity of the endometrium and embryos. Myometrial slices were collected from gravid and nongravid pigs on days 10 or 11, 12 or 13 and 15 or 16 and studied for: (1) the relative abundance of IGF-1R and EGFR mRNA transcripts and proteins, to determine myometrial readiness to response growth factors treatment and (2) the effect of IGF-1 or EGF on the myometrial release of androstenedione (A₄), testosterone (T), estrone (E₁) and estradiol-17β (E₂). The results showed that the relative expression and abundance of IGF-1R and EGFR in the myometrium were altered regarding the female reproductive status. During the estrous cycle, EGF increased myometrial release of A₄ on days 12–13 and E₂ on days 15–16. In gravid pigs (days 15–16), IGF-1 and EGF increased the E₁ release. In conclusion: (1) porcine myometrium possesses the potential to respond to IGF-1 and EGF treatment, (2) EGF significantly increases myometrial A₄ and E₂ release in cyclic pigs, while IGF-1 and EGF increase the E₁ release in gravid pigs.

中文翻译：

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胰岛素样生长因子1（IGF-1）和表皮生长因子（EGF）受体的表达以及IGF-1和EGF对猪子宫内膜雄激素和雌激素释放的影响。

猪子宫肌层具有类固醇生成活性，但其调控尚不清楚。有人认为，子宫肌层类固醇生成的调节剂是胰岛素样生长因子1（IGF-1）和表皮生长因子（EGF），它们可调节子宫内膜和胚胎的类固醇生成活性。在第10或11、12、13或15或16天从妊娠猪和非重力猪收集肌层切片，并研究：（1）IGF-1R和EGFR mRNA转录本和蛋白质的相对丰度，以确定肌层对反应生长的准备情况。因子治疗和（2）IGF-1或EGF对雄烯二酮（A ₄），睾丸激素（T），雌酮（E ₁）和雌二醇-17β（E ₂）的子宫肌层释放的影响）。结果表明，女性生殖状态改变了子宫内膜中IGF-1R和EGFR的相对表达和丰度。在发情周期中，EGF在12-13天增加肌层A ₄释放，在15-16天增加E ₂。在妊娠猪（第15-16天）中，IGF-1和EGF增加了E _1的释放。总之：（1）猪子宫肌层具有潜力来响应IGF-1和EGF处理，（2）EGF显著增加肌层甲₄和E ₂释放在环状猪，而IGF-1和EGF增加对E ₁在释放妊娠猪。