Although the externally controllable extracellular supply of the short‐lived reactive oxygen and nitrogen species, such as O₂^•−, ^•NO, and ONOO⁻, could potentially manipulate cellular functions, their simple administration to cells is likely to be ineffective due to their rapid deactivation. In this study, we found a method of a continuous supply of O₂^•−/ONOO⁻ over a few minutes, which is triggered by irradiation of a nonequilibrium atmospheric pressure plasma to commonly used organic buffers (e.g., 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid, HEPES). In addition, a continuous low‐dose O₂^•−/ONOO⁻ supply was shown to induce a physiologically relevant Ca²⁺ response and subsequently the uptake of a large cation mediated by transient receptor potential channel family member(s). Our results provide a novel approach to the continuous O₂^•−/ONOO⁻ supply, requiring controllable and mass‐volume treatments.

中文翻译：

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血浆暴露的HEPES缓冲盐水中O2- / ONOO-的持续释放促进了TRP通道介导的大阳离子的吸收

虽然短暂的活性氧和氮物种的外部可控的细胞外供应，如O ₂ ^{• -} ，^• NO，和ONOO ^- ，可能操纵的细胞功能，它们的简单施用给细胞很可能是无效的，因为它们快速停用。在这项研究中，我们发现的连续供给的方法O ₂ ^{• -} / ONOO ^-在几分钟内，它是由非平衡大气压等离子体照射触发以通常使用的有机缓冲液（例如，4-（2-羟基乙基）-1-哌嗪乙磺酸（HEPES）。另外，一个连续低剂量ö ₂ ^{• -} / ONOO ^-结果表明，钙供应诱导了生理上相关的Ca ²⁺反应，并随后吸收了由瞬时受体电位通道家族成员介导的大阳离子。我们的研究结果提供一种新颖的方法来连续Ò ₂ ^{• -} / ONOO ^-供应，需要可控的和质量-体积治疗。