Research question

Sex hormone-binding globulin (SHBG), androgen receptor (AR), LH beta polypeptide (LHB), progesterone receptor membrane component 1 (PGRMC1) and progesterone receptor membrane component 2 (PGRMC2) regulate follicle development and maturation. Their mRNA expression was assessed in peripheral blood mononuclear cells (PBMC) of normal and poor responders, during ovarian stimulation.

Design

Fifty-two normal responders and 15 poor responders according to the Bologna criteria were enrolled for IVF and intracytoplasmic sperm injection and stimulated with 200 IU of follitrophin alpha and gonadotrophin-releasing hormone antagonist. HCG was administered for final oocyte maturation. On days 1, 6 and 10 of stimulation, blood samples were obtained, serum hormone levels were measured, RNA was extracted from PBMC and real-time polymerase chain reaction was carried out to identify the mRNA levels. Relative mRNA expression of each gene was calculated by the comparative 2^−DDCt method.

Results

Differences between mRNA levels of each gene on the same time point between the two groups were not significant. PGRMC1 and PGRMC2 mRNA levels were downregulated, adjusted for ovarian response and age. Positive correlations between PGRMC1 and AR (standardized beta = 0.890, P < 0.001) from day 1 to 6 and PGRMC1 and LHB (standardized beta = 0.806, P < 0.001) from day 1 to 10 were found in poor responders. PGRMC1 and PGRMC2 were positively correlated on days 6 and 10 in normal responders.

Conclusions

PGRMC1 and PGRMC2 mRNA are significantly decreased during ovarian stimulation, with some potential differences between normal and poor responders.

中文翻译：

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低反应者卵巢刺激过程中调节卵泡发育和成熟的基因表达

研究问题

性激素结合球蛋白 ( SHBG )、雄激素受体 ( AR )、LH β 多肽 ( LHB )、孕激素受体膜成分 1 ( PGRMC1 ) 和孕激素受体膜成分 2 ( PGRMC2 ) 调节卵泡发育和成熟。在卵巢刺激期间，在正常和反应不佳者的外周血单核细胞 (PBMC) 中评估了它们的 mRNA 表达。

设计

根据博洛尼亚标准，52 名正常反应者和 15 名反应差者被纳入 IVF 和卵胞浆内单精子注射，并用 200 IU 促卵泡激素 α 和促性腺激素释放激素拮抗剂刺激。HCG 用于最终的卵母细胞成熟。在刺激的第 1、6 和 10 天，采集血样，测量血清激素水平，从 PBMC 中提取 RNA，并进行实时聚合酶链反应以鉴定 mRNA 水平。通过比较2- ^DDCt方法计算每个基因的相对mRNA表达。

结果

两组间同一时间点各基因mRNA水平差异不显着。PGRMC1和PGRMC2 mRNA 水平下调，根据卵巢反应和年龄进行调整。在反应差的人中发现了第 1天到第 6 天的PGRMC1和AR（标准化β = 0.890，P < 0.001）与第 1天到第 10 天的PGRMC1和LHB（标准化β = 0.806，P < 0.001）之间的正相关。PGRMC1和PGRMC2在正常反应者的第 6 天和第 10 天呈正相关。

结论

PGRMC1和PGRMC2 mRNA 在卵巢刺激过程中显着降低，正常反应者和反应差者之间存在一些潜在差异。