Vernonia anthelmintica (L.) Willd. belongs to the family Asteraceae and has anthelmintic, anti-diabetic, diuretic and anti-asthmatic properties. Present study describes the production of rhamnetin: an O-methylated flavonol with anti-oxidant and anti-inflammatory activities; in cell suspension cultures of V. anthelmintica. Suspension cultures were established from cream coloured friable calli initiated from leaf explants and cultured in liquid MS medium supplemented with combinations of IAA (1.5 mg L⁻¹) and BAP (1.5 mg L⁻¹). After 20 days of cell suspension culture, the initial inoculum size of 0.5 g was increased to 1.05 g 100 mL⁻¹ dry weight at exponential phase. When the culture was treated with 0.8 mM methyl jasmonate for 6 days; 2.2 fold accumulation of rhamnetin production was obtained on a dry weight basis compared to unelicited control. Likewise, the elicitation with salicylic acid 1 mM for 6 days brought about 2.7 fold rhamnetin production compared to unelicited control. The rhamnetin content in dried leaf tissue of field grown plants was found to be 0.143 mg g⁻¹. Elicitation with methyl jasmonate and salicylic acid brought about 7.7 and 9.4 fold increments in rhamnetin production, compared to leaf tissue of field grown plants on a dry weight basis.

中文翻译：

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驱虫斑鸠菊中鼠李素产量的提高。通过用茉莉酸甲酯和水杨酸诱导来进行细胞悬浮培养。

驱虫斑鸠菊(L.) Willd。属于菊科，具有驱虫、抗糖尿病、利尿和抗哮喘特性。目前的研究描述了鼠李素的生产：一种具有抗氧化和抗炎活性的 O-甲基化黄酮醇；在驱虫弧菌的细胞悬浮培养物中。^{悬浮培养物是从叶外植体开始的奶油色易碎愈伤组织建立的，并在补充有IAA (1.5 mg L -1} )和BAP (1.5 mg L ^-1 )组合的液体MS培养基中培养。细胞悬浮培养20天后，初始接种量0.5g在指数生长期增加至1.05g 100mL ^-1干重。当用0.8 mM茉莉酸甲酯处理培养物6天时；与未引出的对照相比，以干重计获得了2.2倍的鼠李素产量积累。同样，与未引发的对照相比，用 1 mM 水杨酸引发 6 天，鼠李素产量增加了约 2.7 倍。发现田间生长的植物的干燥叶组织中鼠李素含量为0.143 mg g ^-1。与田间种植植物的叶组织干重相比，用茉莉酸甲酯和水杨酸诱导可使鼠李素产量增加约 7.7 和 9.4 倍。