Abstract

In this work, we study the mechanism of peptide photodissociation by ultraviolet irradiation at 193 nm wavelength and discuss the role of ionization proton in this process. We found that substituting the ionization proton for the alkali metal cations (sodium) in the peptide ions results in significant changes of the photodissociation spectra. The experimental data obtained in this work revealed that the photodissociation process can be described using the mobile proton model introduced earlier for peptide collision dissociation. The results can be used in proteomics research for optimization of mass spectrometer’s parameters to increase the efficiency of peptide dissociation and in developing sequence-specific models for peptide fragmentation prediction.

中文翻译：

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肽的紫外光解离：移动质子模型的新见解

摘要

在这项工作中，我们研究了在193 nm波长下通过紫外线辐照引起的肽光解离的机理，并讨论了电离质子在此过程中的作用。我们发现，用电离质子取代肽离子中的碱金属阳离子（钠）会导致光解离光谱发生重大变化。在这项工作中获得的实验数据表明，可以使用较早引入的用于肽碰撞解离的移动质子模型来描述光解离过程。该结果可用于蛋白质组学研究，以优化质谱仪的参数以提高肽解离的效率，并可用于开发序列特异性模型以预测肽片段化。