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Short-Term Environmental Enrichment is a Stronger Modulator of Brain Glial Cells and Cervical Lymph Node T Cell Subtypes than Exercise or Combined Exercise and Enrichment.
Cellular and Molecular Neurobiology ( IF 4 ) Pub Date : 2020-05-25 , DOI: 10.1007/s10571-020-00862-x
Gaurav Singhal 1 , Julie Morgan 1 , Frances Corrigan 2 , Catherine Toben 1 , Magdalene C Jawahar 1 , Emily J Jaehne 1, 3 , Jim Manavis 4 , Anthony J Hannan 5 , Bernhard T Baune 5, 6, 7
Affiliation  

Physical exercise (PE) and environmental enrichment (EE) can modulate immunity. However, the differential effects of short-term PE, EE, and PE + EE on neuroimmune mechanisms during normal aging has not been elucidated. Hence, a cohort of 3-, 8-, and 13-month-old immunologically unchallenged C57BL/6 wild-type mice were randomly assigned to either Control, PE, EE, or PE + EE groups and provided with either no treatment, a running wheel, a variety of plastic and wooden objects alone or in combination with a running wheel for seven weeks, respectively. Immunohistochemistry and 8-color flow cytometry were used to determine the numbers of dentate gyrus glial cells, and the proportions of CD4+ and CD8+ T cell numbers and their subsets from cervical lymph nodes, respectively. An increase in the number of IBA1+ microglia in the dentate gyrus at 5 and 10 months was observed after EE, while PE and PE + EE increased it only at 10 months. No change in astroglia number in comparison to controls were observed in any of the treatment groups. Also, all treatments induced significant differences in the proportion of specific T cell subsets, i.e., CD4+ and CD8+ T naïve (TN), central memory (TCM), and effector memory (TEM) cells. Our results suggest that in the short-term, EE is a stronger modulator of microglial and peripheral T cell subset numbers than PE and PE + EE, and the combination of short-term PE and EE has no additive effects.



中文翻译:

短期环境富集是一种比锻炼或联合锻炼和富集更强大的脑胶质细胞和颈淋巴结 T 细胞亚型调节剂。

体育锻炼 (PE) 和丰富环境 (EE) 可以调节免疫力。然而,短期 PE、EE 和 PE + EE 对正常衰老过程中神经免疫机制的不同影响尚未阐明。因此,一组 3 个月、8 个月和 13 个月大的免疫学未受攻击的 C57BL/6 野生型小鼠被随机分配到 Control、PE、EE 或 PE + EE 组,并提供不治疗、跑轮,各种塑料和木制物品单独使用或与跑轮结合使用,分别为七周。免疫组化和8色流式细胞术测定齿状回神经胶质细胞数、CD4 +和CD8 +比例分别来自颈部淋巴结的 T 细胞数量及其子集。EE 后 5 个月和 10 个月观察到齿状回中IBA1 +小胶质细胞的数量增加,而 PE 和 PE + EE 仅在 10 个月时增加。与对照组相比,在任何治疗组中都没有观察到星形胶质细胞数量的变化。此外,所有治疗都诱导了特定 T 细胞亚群比例的显着差异,即 CD4 +和 CD8 + T naïve (T N )、中央记忆 (T CM ) 和效应记忆 (T EM) 细胞。我们的结果表明,在短期内,EE 是比 PE 和 PE + EE 更强的小胶质细胞和外周 T 细胞亚群数量调节剂,并且短期 PE 和 EE 的组合没有累加效应。

更新日期:2020-05-25
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