Obligate symbiotic associations are present in a wide variety of animals with a nutrient-restricted diet. Aphids (hemiptera: Aphididae) almost-universally host Buchnera aphidicola bacteria in specialised organs (called bacteriomes). These bacteria supply the aphid with essential nutrients lacking from their diet (i.e. essential amino acids and some B vitamins). Some aphid lineages, such as species from the Lacninae subfamily, have evolved co-obligate associations with secondary endosymbionts, deriving from a loss of biotin- and riboflavin-biosynthetic genes. In this study, I re-analyse previously published sequencing data from the banana aphid Pentalonia nigronervosa. I show that the metabolic inference results from De Clerck et al. (Microbiome 3:63, 2015) are incorrect and possibly arise from the use of inadequate methods. Additionally, I discuss how the seemingly biased interpretation of their antibiotic treatment analyses together with an incorrect genome-based metabolic inference resulted in the erroneous suggestion "that a co-obligatory symbiosis between B. aphidicola and Wolbachia occurs in the banana aphid".

中文翻译：

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没有证据表明Wolbachia在蚜虫Pentalonia nigronervosa中是一种营养共生内共生体。

限制营养饮食的多种动物中存在专性共生联系。蚜虫（半翅目：Aphididae）几乎在特定器官中都存在Buchnera Aphidicola细菌（称为细菌群）。这些细菌为蚜虫提供饮食中缺乏的必需营养素（即必需氨基酸和某些B族维生素）。一些蚜虫谱系，例如来自网藻亚科的物种，已经与次生内共生体形成了共专一的联系，这是由于生物素和核黄素生物合成基因的丧失而引起的。在这项研究中，我重新分析了香蕉蚜虫Pentalonia nigronervosa先前发表的测序数据。我表明De Clerck等人的代谢推断结果。（Microbiome 3:63，2015）是不正确的，可能是由于使用方法不当引起的。另外，