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Democratising "Microscopi": a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork
bioRxiv - Scientific Communication and Education Pub Date : 2020-12-22 , DOI: 10.1101/2020.05.21.108894
Matthew Wincott , Andrew Jefferson , Ian M. Dobbie , Martin J. Booth , Ilan Davis , Richard M. Parton

Commercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing Microscopi, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens.

中文翻译:

民主化“ Microscopi”:3D打印的自动XYZT荧光成像系统,用于教学,外展和野外工作

商业荧光显微镜支架和全自动XYZt荧光成像系统通常超出了用于教学和推广的有限预算。我们已经通过开发Microscopi解决了这个问题,Microscopi是一种可访问的,价格合理的DIY自动化成像系统,它是由3D打印和商品现成的硬件(包括机电,计算机和光学组件)构建的。我们的设计具有一个简单的基于Web的图形用户界面,可通过平板电脑或其他移动设备进行访问,从而实现自动样品导航和图像捕获。可以轻松地在不同的成像模式之间切换光路。基于Python的开源控制软件允许将硬件作为集成的映像系统进行驱动。此外,显微镜是完全可定制的,这也增强了其作为学习工具的价值。在这里,我们描述了基本设计并展示了一系列易于获取标本的成像性能。
更新日期:2020-12-23
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