Various mixtures were prepared depending on the mixing ratio of Scutellaria baicalensis hot water extract (SB-HW), and Chrysanthemum morifolium ethanol extract (CM-E) and their anti-inflammatory activity were compared. Among them, SB-HW (80 μg/mL)/CM-E (120 μg/mL) or SB-HW (40 μg/mL)/CM-E (160 μg/mL) significantly inhibited LPS-stimulated NO and IL-6 levels in RAW 264.7 cells. The SB-HW (80 μg/mL)/CM-E (120 μg/mL) mixture, which was determined as active mixture, significantly reduced MUC5AC secretion in PMA and LPS-induced NCI-H292 cells. The active mixture also reduced the production of PGE₂ and IL-8 in PMA-induced A549 cells. LC-MS/MS analysis showed that the active mixture was composed of high contents of flavone glycosides, such as baicalin and cynaroside. Western blot analysis indicated that the active mixture suppressed phosphorylation of ERK, JNK, and p38, associating with the inhibition of MAPK signaling. Taken together, our results suggest that the active mixture could be applied as a new anti-inflammatory herbal medicine.

Abbreviations

JNK: c-Jun N-terminal kinases; COPD: chronic obstructive pulmonary disease; CM: Chrysanthemum morifolium; COX-2: cyclooxygenase-2; ERK: extracellular-signal-regulated kinase; IL-6: interleukin-6; IL-8: interleukin-8; IL-12: interleukin-12; LPS: lipopolysaccharide; MAPK: mitogen-activated protein kinase; NO: nitric oxide; NK- κB: nuclear factor kappa B; p38: p38 mitogen-activated protein kinases; PBS: phosphate buffered saline; PMA: phorbol-12-myristate-13-acetate; SB: Scutellaria baicalensis; PGE₂: prostaglandin E2; TBST: Tris-buffered saline containing 0.1% Tween 20; TIC: total ion chromatogram; TNF-α: tumor necrosis factor-alpha

中文翻译：

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包含黄S和菊花的草药的抗炎作用。

根据黄cut热水提取物（SB-HW）和菊花mo乙醇提取物（CM-E）的混合比例制备各种混合物，并比较它们的抗炎活性。其中SB-HW（80μg/ mL）/ CM-E（120μg/ mL）或SB-HW（40μg/ mL）/ CM-E（160μg/ mL）显着抑制LPS刺激的NO和IL RAW 264.7细胞中的-6水平。被确定为活性混合物的SB-HW（80μg/ mL）/ CM-E（120μg/ mL）混合物显着降低了PMA和LPS诱导的NCI-H292细胞中MUC5AC的分泌。活性混合物还减少了PGE _2的产生和PMA诱导的A549细胞中的IL-8。LC-MS / MS分析表明，活性混合物由高含量的黄酮糖苷，如黄in苷和西诺糖苷组成。蛋白质印迹分析表明，活性混合物抑制ERK，JNK和p38的磷酸化，并与MAPK信号传导的抑制有关。两者合计，我们的结果表明，该活性混合物可以用作一种新型的消炎草药。

缩略语

JNK：c-Jun N-末端激酶；COPD：慢性阻塞性肺疾病；CM：菊花。COX-2：环氧合酶-2；ERK：细胞外信号调节激酶；IL-6：白介素6；IL-8：白介素8；IL-12：白介素12；LPS：脂多糖；MAPK：促分裂原活化蛋白激酶；NO：一氧化氮；NK-κB：核因子κB；p38：p38有丝分裂原激活的蛋白激酶；PBS：磷酸盐缓冲盐水；PMA：佛波醇12-肉豆蔻酸酯13-醋酸酯；SB：黄cut；PGE ₂：前列腺素E ₂；TBST：含0.1％Tween 20的Tris缓冲盐水；TIC：总离子色谱图；TNF-α：肿瘤坏死因子-α