Many phenolic compounds, derived from lignin during the pretreatment of lignocellulosic biomass, could obviously inhibit the activity of cellulolytic and hemicellulolytic enzymes. Acetosyringone (AS) is one of the phenolic compounds produced from lignin degradation. In this study, we investigated the inhibitory effects of AS on xylanase activity through kinetic experiments. The results showed that AS could obviously inhibit the activity of xylanase in a reversible and noncompetitive binding manner (up to 50% activity loss). Inhibitory kinetics and constants of xylanase on AS were conducted by the HCH-1 model (β = 0.0090 ± 0.0009 mM⁻¹). Furthermore, intrinsic and 8-anilino-1-naphthalenesulfonic (ANS)-binding fluorescence results showed that the tertiary structure of AS-mediated xylanase was altered. These findings provide new insights into the role of AS in xylanase activity. Our results also suggest that AS was an inhibitor of xylanase and targeting AS was a potential strategy to increase xylose production.

在木质纤维素生物质的预处理过程中，木质素衍生的许多酚类化合物可以明显抑制纤维素分解酶和半纤维素分解酶的活性。乙酰丁香酮（AS）是木质素降解产生的酚类化合物之一。在这项研究中，我们通过动力学实验研究了AS对木聚糖酶活性的抑制作用。结果表明，AS可以以可逆和非竞争性的结合方式明显抑制木聚糖酶的活性（活性损失高达50％）。用HCH-1模型进行木聚糖酶对AS的抑制动力学和常数（β  = 0.0090±0.0009 mM ^-1）。此外，固有的和8-苯胺基-1-萘磺酸（ANS）结合荧光结果表明，AS介导的木聚糖酶的三级结构发生了变化。这些发现为AS在木聚糖酶活性中的作用提供了新的见解。我们的结果还表明，AS是木聚糖酶的抑制剂，而靶向AS是增加木糖产量的潜在策略。