The presence of biological cues to promote the attachment, proliferation, and differentiation of neuronal cells is important in the process of nerve regeneration. In this study, laminin as a neurite promoting protein, has been used to modify poly‐lactide‐co‐glycolide/carbon nanotube (PLGA/CNT) electrospun nanofibrous scaffolds by means of either mussel‐inspired poly(dopamine) (PD) coating or via direct physical adsorption as a simple route for the functionalization of biomaterials. The laminin‐modified scaffolds were characterized by a combination of field emission scanning electron microscopy (SEM), X‐ray photoelectron spectroscopy, and contact angle measurements. Subsequently, various properties of scaffolds such as degradation time, amount of attached laminin and the rate of CNT release were investigated. The synergistic effect of topographical and biological cues for PC12 cell attachment, proliferation, and differentiation were then studied by SEM and confocal microscopy. The results of degradation study showed that laminin‐modified scaffolds were biodegradable with good structural integrity that persisted about 4 weeks. The amount of laminin attached to the PLGA/CNT and PLGA/CNT‐PD scaffolds was 3.12 ± 0.6 and 3.04 ± 071 μg per mg of the scaffold, respectively. Although laminin‐modified scaffolds could improve cell proliferation identically, neurite extensions on the PLGA/CNT scaffold modified via PD coating (PLGA/CNT‐PD‐lam scaffold) were significantly longer than those observed on PLGA/CNT scaffold modified via physical adsorption (PLGA/CNT‐lam scaffold) and unmodified scaffolds. Together, these results indicated that surface modification via PD coating could be a promising strategy to fabricate biomimetic scaffolds capable of sustaining longer neuronal growth for nerve tissue engineering.

中文翻译：

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层粘连蛋白对聚丙交酯-共-乙交酯/碳纳米管纳米纤维支架表面改性对神经组织工程的影响。

促进神经元细胞附着、增殖和分化的生物学线索的存在在神经再生过程中很重要。在这项研究中，层粘连蛋白作为轴突促进蛋白质，已被用于修改聚丙交酯共-乙交酯/碳纳米管（PLGA/CNT）电纺纳米纤维支架，通过受贻贝启发的聚（多巴胺）（PD）涂层或通过直接物理吸附作为生物材料功能化的简单途径。层粘连蛋白修饰的支架通过场发射扫描电子显微镜 (SEM)、X 射线光电子能谱和接触角测量的组合进行表征。随后，研究了支架的各种特性，例如降解时间、附着的层粘连蛋白的量和 CNT 的释放速率。然后通过扫描电镜和共聚焦显微镜研究了地形和生物线索对 PC12 细胞附着、增殖和分化的协同作用。降解研究结果表明，层粘连蛋白修饰的支架是可生物降解的，具有良好的结构完整性，可持续约 4 周。附着在 PLGA/CNT 和 PLGA/CNT-PD 支架上的层粘连蛋白的量分别为 3.12 ± 0.6 和 3.04 ± 071 μg/mg 支架。尽管层粘连蛋白修饰的支架可以同样提高细胞增殖，但通过 PD 涂层修饰的 PLGA/CNT 支架（PLGA/CNT-PD-lam 支架）上的轴突延伸明显长于通过物理吸附修饰的 PLGA/CNT 支架（PLGA /CNT-lam 支架）和未改性的支架。总之，这些结果表明，通过 PD 涂层进行表面改性可能是一种很有前景的策略，可以制造能够维持神经组织工程神经元生长时间更长的仿生支架。