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Genetic Code Expansion Facilitates Position-Selective Modification of Nucleic Acids and Proteins.
ChemPlusChem ( IF 3.4 ) Pub Date : 2020-05-22 , DOI: 10.1002/cplu.202000150
Diana Müller 1 , Sven Trucks 1 , Harald Schwalbe 1 , Martin Hengesbach 1
Affiliation  

Transcription and translation obey to the genetic code of four nucleobases and 21 amino acids evolved over billions of years. Both these processes have been engineered to facilitate the use of non‐natural building blocks in both nucleic acids and proteins, enabling researchers with a decent toolbox for structural and functional analyses. Here, we review the most common approaches for how labeling of both nucleic acids as well as proteins in a site‐selective fashion with either modifiable building blocks or spectroscopic probes can be facilitated by genetic code expansion. We emphasize methodological approaches and how these can be adapted for specific modifications, both during as well as after biomolecule synthesis. These modifications can facilitate, for example, a number of different spectroscopic analysis techniques and can under specific circumstances even be used in combination.

中文翻译:

遗传密码扩展方便了核酸和蛋白质的位置选择修饰。

转录和翻译遵循了数十亿年进化出的四个核碱基和21个氨基酸的遗传密码。这两个过程都经过精心设计,可以促进核酸和蛋白质中非天然结构单元的使用,从而使研究人员拥有不错的工具箱来进行结构和功能分析。在这里,我们回顾了最常见的方法,以了解如何通过遗传密码扩展方便地使用可修饰的结构单元或光谱探针以位点选择性方式标记核酸和蛋白质。我们强调方法论方法,以及如何在生物分子合成过程中以及之后对这些方法进行特定修饰。这些修改可以促进例如
更新日期:2020-05-22
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