Sporadic outbreaks of Seneca Valley virus (SVV) have been detected in recent years causing huge economic losses to the pig industry. SVV infection can lead to redness and fever of the mouth, nose or hoof wall, ulcerative injury and inflammation in pigs. Although long non-coding RNAs (lncRNAs) have been shown to play an important role in antiviral and inflammatory regulation, how lncRNAs regulate and induce SVV infection inflammation remains unclear. Here, we found the differential expression of 1332 lncRNAs and 3299 mRNAs in SVV-infected ST cells using RNA-seq. Functional annotation analysis revealed that regulated transcripts are mainly involved in signaling pathways related to host immunity and inflammatory responses. We identified lnc-MSTRG.18940.1 as an important immune regulator in SVV infection. Lnc-MSTRG.18940.1 silencing specifically inhibited SVV replication and the production of inflammatory factors TNF-α, IL-1, IL-6, and IL-8. Our findings aid to a better understanding of host responses to SVV infection and provide new directions for understanding the potential association between lncRNAs and SVV pathogenesis.

近年来发现了塞内卡谷地病毒（SVV）的零星爆发，给养猪业造成了巨大的经济损失。SVV感染可导致猪嘴，鼻或蹄壁发红和发烧，溃疡性损伤和炎症。尽管长的非编码RNA（lncRNA）已显示在抗病毒和炎性调节中起重要作用，但仍不清楚lncRNA如何调节和诱导SVV感染发炎。在这里，我们发现使用RNA序列在SVV感染的ST细胞中有1332个lncRNA和3299 mRNA的差异表达。功能注释分析显示，受调控的转录本主要参与与宿主免疫和炎症反应相关的信号传导途径。我们确定lnc-MSTRG.18940.1是SVV感染中的重要免疫调节剂。Lnc-MSTRG.18940。1沉默可以特异性抑制SVV复制和炎症因子TNF-α，IL-1，IL-6和IL-8的产生。我们的发现有助于更好地理解宿主对SVV感染的反应，并为了解lncRNA与SVV发病机理之间的潜在联系提供新的方向。