Purpose

We studied subcutaneous white adipose tissue (sWAT) of obese mice submitted to intermittent fasting (IF).

Methods

Twelve-week-old C57BL/6 male mice received the diets Control (C) or high-fat (HF) for eight weeks (n = 20/each). Then, part of each group performed IF (24 h feeding/24 h fasting) for four weeks: C, C-IF, HF, and HF-IF (n = 10/each).

Results

Food intake did not show a difference in feeding and fasting days, but HF groups had a high energy intake. IF led to multilocular adipocytes in sWAT (browning), and improved respiratory quotient on the fed day. IF decreased gene expression of Leptin, but increased Adiponectin, β3ar (beta3 adrenoreceptor), and Ucp1 (uncoupling protein). IF enhanced immunostaining of Caspase 3, Pcna (proliferating cell nuclear antigen), and UCP1 in sWAT. IF attenuated pro-inflammatory markers and pro-apoptotic markers in sWAT.

Conclusions

IF in obese mice led to browning in sWAT adipocytes, enhanced thermogenesis, an improved adipose tissue pro-inflammatory profile.

中文翻译：

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饮食诱导的肥胖小鼠的皮下脂肪细胞褐变呈间歇性禁食。

目的

我们研究了间歇性禁食（IF）的肥胖小鼠的皮下白色脂肪组织（sWAT）。

方法

十二周大的C57BL / 6雄性小鼠接受了对照（C）或高脂（HF）饮食八周（n = 20 /每只）。然后，每组中的一部分进行IF（喂食24小时/禁食24小时）四个星期：C，C-IF，HF和HF-IF（n = 10 /每组）。

结果

食物摄入量在进食和禁食天数上没有差异，但是HF组的能量摄入量很高。IF在sWAT中导致多眼脂肪细胞（褐变），并在进食日改善了呼吸商。IF降低了瘦素的基因表达，但增加了脂联素，β3ar（β3肾上腺素受体）和Ucp1（解偶联蛋白）。IF增强了sWAT中Caspase 3，Pcna（增殖细胞核抗原）和UCP1的免疫染色。IF减弱了sWAT中的促炎标记和促凋亡标记。

结论

肥胖小鼠的中频导致sWAT脂肪细胞褐变，热生成增强，脂肪组织促炎性改善。