Investigating the interaction between insect pests and host plants at molecular level, provides an insight for effective implementation of plant resistance-based strategies in insect pest control programs. Heat shock proteins (HSPs) are found in all living organisms which protect them from biotic and abiotic stresses. Stress-activated protein kinases (SAPKs) are members of MPK kinases which also are activated upon cellular stresses. We investigated the effect of seed-primed canola plants with salicylic acid (SA) and the plants treated with dilute sulfuric acid on the regulation of two stress-related genes, HSP70 and SAPK/JNK genes in the cabbage aphid. The results of qRT-PCR revealed a statistically significant up-regulation of HSP70 gene expression in aphids feeding on sulfuric acid-treated plants in comparison with controls (P < 0.01). However, this gene was not regulated in aphids feeding on salicylic acid-treated plants. In addition, quantification of SAPK/JNK gene showed a significant effect in aphids feeding on SA-primed plants (P < 0.05) and a marginally significant effect in sulfuric acid treatment. Further, the expression of both genes were significantly changed in interaction effect between SA and sulfuric acid treatments. Finally, the induction of HSP70 and SAPK/JNK genes in this research might be related to activation of systemic acquired resistance in canola.

在分子水平上研究害虫与寄主植物之间的相互作用，为在害虫控制计划中有效实施基于植物抗性的策略提供了见识。在所有活生物体中都发现了热激蛋白（HSP），可以保护它们免受生物和非生物胁迫。应激激活的蛋白激酶（SAPKs）是MPK激酶的成员，其在细胞应激时也会被激活。我们调查了用水杨酸（SA）接种种子的低芥酸菜籽植物和用稀硫酸处理的植物对甘蓝蚜中与胁迫相关的两个基因HSP70和SAPK / JNK基因的调控。qRT-PCR结果显示HSP70在统计学上显着上调与对照相比，以硫酸处理过的植物为食的蚜虫中的基因表达（P <0.01）。但是，该基因在以水杨酸处理过的植物为食的蚜虫中没有受到调控。此外，对SAPK / JNK基因的定量显示在蚜虫饲喂SA致敏植株的蚜虫中有显着效果（P <0.05），在硫酸处理中也有显着影响。此外，两个基因的表达在SA和硫酸处理之间的相互作用中显着改变。最后，本研究中HSP70和SAPK / JNK基因的诱导可能与油菜中系统获得性抗药性的激活有关。