Food Bioscience ( IF 5.2 ) Pub Date : 2020-05-23 , DOI: 10.1016/j.fbio.2020.100653 Leandro P. Ribeiro , Vanessa C. Domingues , Gabriel L.P. Gonçalves , João B. Fernandes , Eduardo M. Glória , José D. Vendramim
To search for new compounds with promising grain-protective properties, this study evaluated the chemical composition and insecticidal, antifungal and antiaflatoxigenic activities of essential oil (EO) from the hydrodistillation of Duguetia lanceolata (Annonaceae) leaves on fungi and insects that spoil stored commodities. By combining the chromatographic and spectroscopic analyses, 5 compounds were identified in the EO of D. lanceolata leaves, including the major compounds β-bisabolene (56.2%) and 2,4,5-trimethoxystyrene (19.1%). This EO showed promising lethal effects on adult Sitophilus zeamais (LC50 = 457 mg kg−1) and Zabrotes subfasciatus (LC50 = 442 mg kg−1) using a residual contact bioassay. In addition to lethal effects, the EO strongly reduced the number of adult S. zeamais that emerged from the treated samples (EC50 = 480 mg kg−1), the percentage of damaged grains (EC50 = 543 mg kg−1) and the sample weight loss (EC50 = 543 mg kg−1). For Z. subfasciatus, D. lanceolata EO significantly reduced the number of eggs/sample (EC50 = 118 mg kg−1), the number of emerged insects (F1 progeny; EC50 = 119 mg kg−1) and the percentage of damaged grains (EC50 = 148 mg kg−1). However, the EO led to no significant mortality in adults of either species using fumigation. The EO also inhibited radial growth and aflatoxin B1 production in the CCT7638 Aspergillus flavus isolate (Ascomycota) in a concentration-dependent manner. Thus, the EO of D. lanceolata leaves may be a promising source of compounds with potential grain-protective properties.
中文翻译:
来自Duguetia lanceolata St.- Hil的精油。(Annonaceae):抑制储存谷物的破坏
为了寻找具有前景的谷类保护特性的新化合物,本研究评估了杜仲叶(Annueaceae)叶经真菌和昆虫的加氢蒸馏后精制而成的植物的化学成分以及精油(EO)的杀虫,抗真菌和抗黄曲霉活性。通过色谱和光谱分析相结合,在D. lanceolata叶的EO中鉴定出5种化合物,包括主要化合物β-bisabolene(56.2%)和2,4,5-三甲氧基苯乙烯(19.1%)。该EO对成年玉米(Sitophilus zeamais)(LC 50 = 457 mg kg -1)和Zabrotes subs fasciatus(LC 50)具有致命的杀伤作用 = 442 mg kg -1),采用残留接触生物测定法。除了致命的影响外,EO还大大减少了从处理过的样品中出现的成年玉米(S. zeamais)数量(EC 50 = 480 mg kg -1),受损谷物的百分比(EC 50 = 543 mg kg -1)和样品失重(EC 50 = 543 mg kg -1)。为Z. subfasciatus,D.杉木EO蛋的数量减少显著/样品(EC 50 = 118毫克千克-1),出现的昆虫的数量(F 1个子代; EC 50 = 119毫克千克-1)和受损谷物的百分比(EC 50 = 148 mg kg -1)。但是,使用熏蒸法,EO不会导致这两种物种的成年动物死亡。EO还以浓度依赖的方式抑制CCT7638黄曲霉分离株(Ascomycota)中的径向生长和黄曲霉毒素B 1的产生。因此,D。lanceolata叶的EO可能是具有潜在谷物保护特性的化合物的有希望的来源。