This study applies individual and combined application of ultrasound and ozone pretreatments for the tandem recovery of microalgae biocompounds (lipids, proteins, and, carbohydrates) and phosphorus from Scenedesmus obliquus cultivated in wastewater. Results of individual application showed that ultrasound released protein to the aqueous phase (99 ± 3 %), and increased microalgal lipid viability from 11 to 90 ± 2 %. Whilst ozone pretreatment induced carbohydrate release to the aqueous phase 28 ± 1 %. Combined ultrasound and ozone pretreatment were studied in sequence mode. Ultrasound was first applied to release proteins and expose lipids, followed by ozone to induce carbohydrates and phosphorus release. The maximum yields of recovered lipids, proteins, carbohydrates and phosphorus by the combined pretreatment were 89 ± 1 %, 91 ± 8 %, 63 ± 1 %, and 75 ± 1 % respectively. In the dual pretreatment best experimental parameters applied for ultrasound were: biomass concentration of 25 g/L and applied energy of 200 kW h/kg dry biomass. Ozone pretreatment used 27 mg O₃/L, contact time 25 min and pH of 11. The combined pretreatment intensified microalgae fractionation achieving high biocompounds and phosphorus recovery yields; reducing solvent waste by 95 % and extraction time by 90 %, in comparison to biocompounds extraction without pretreatment.

这项研究应用超声波和臭氧预处理的单独和组合应用，以串联回收斜生场景藻中的微藻生物化合物（脂质，蛋白质和碳水化合物）和磷培养在废水中。单独应用的结果表明，超声将蛋白质释放到水相中（99±3％），并使微藻脂质的生存力从11增加到90±2％。臭氧预处理导致碳水化合物释放到水相中为28±1％。以顺序模式研究了超声和臭氧组合预处理。首先使用超声波释放蛋白质并暴露脂质，然后使用臭氧诱导碳水化合物和磷的释放。通过联合预处理，回收的脂质，蛋白质，碳水化合物和磷的最大产率分别为89±1％，91±8％，63±1％和75±1％。在双重预处理中，应用于超声波的最佳实验参数是：生物质浓度为25 g / L，施加的能量为200 kW h / kg干生物质。臭氧预处理使用27 mg O₃ / L，接触时间25分钟，pH值为11。联合预处理可增强微藻的分馏作用，从而实现高生物化合物和磷的回收率；与未经预处理的生物化合物相比，可减少95％的溶剂浪费和90％的提取时间。