Susceptibility profiles of anaerobic bacteria to antibiotics have become unpredictable, thus reliable and user-friendly methods for routine susceptibility testing are needed. In this study, we evaluated the MICRONAUT-S Anaerobes MIC test plate, a commercially available broth microdilution method, and suitable for clinical microbiology routine testing. We analyzed a collection of 300 consecutive clinically significant isolates, including 149 Gram-positive and 151 Gram-negative strains. The performance of the MICRONAUT-S Anaerobes MIC plate was compared to that of a gradient diffusion method (current laboratory standard), calculating the essential and the categorial agreement. 99.7% (299/300) of the strains included in this study successfully grew in the MICRONAUT-S Anaerobes MIC plate (73% of them after 24 h of incubation), while 1 Porphyromonas uenoni isolate didn’t grow. It showed a high concordance with the gradient diffusion method. Overall essential and categorical agreements resulted >95% and >97%, respectively, and only a low rate of errors was observed. Beyond the very good analytical performance, several technical advantages in comparison with the gradient diffusion method were observed, that contribute to make the MICRONAUT-S Anaerobes panels suitable for an easy implementation into laboratory routine.

厌氧细菌对抗生素的敏感性概况已变得不可预测，因此需要可靠且用户友好的常规敏感性测试方法。在这项研究中，我们评估了MICRONAUT-S Anaerobes MIC测试板，这是一种市售的肉汤微量稀释方法，适用于临床微生物常规测试。我们分析了300个连续的临床上重要的分离株的集合，其中包括149株革兰氏阳性和151株革兰氏阴性。将MICRONAUT-S Anaerobes MIC板的性能与梯度扩散法（当前的实验室标准）的性能进行比较，计算出基本和分类协议。这项研究中包括的99.7％（299/300）菌株在MICRONAUT-S Anaerobes MIC平板中成功生长（孵育24小时后其中的73％），而1卟啉单胞菌分离株未生长。与梯度扩散法具有高度的一致性。总体基本和绝对协议分别导致> 95％和> 97％，并且仅观察到较低的错误率。除了非常好的分析性能外，与梯度扩散方法相比，还观察到了一些技术优势，这些优势使MICRONAUT-S Anaerobes面板适用于实验室程序的轻松实现。