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Grape seed proanthocyanidin extract ameliorates hepatic lipid accumulation and inflammation in grass carp (Ctenopharyngodon idella).
Fish Physiology and Biochemistry ( IF 2.9 ) Pub Date : 2020-05-24 , DOI: 10.1007/s10695-020-00819-3
Rong-Hua Lu 1, 2 , Chao-Bin Qin 1 , Feng Yang 1, 2 , Wen-Ya Zhang 1, 2 , Yu-Ru Zhang 1, 2 , Guo-Kun Yang 1 , Li-Ping Yang 1, 2 , Xiao-Lin Meng 1, 2 , Xiao Yan 1, 2 , Guo-Xing Nie 1, 2
Affiliation  

Hepatic lipid metabolism disorder due to excessive fat accumulation in fish is a significant problem in aquaculture. Studies have shown that grape seed procyanidin extract (GSPE) can regulate fish lipid metabolism and improve fish immunity. However, the mechanism is unclear. In this study, we used grass carp that stores excess fat in the liver as a model. In vitro, GSPE treatment of hepatocytes for 3 h significantly decreased TG content, accompanied with decreased expression of SREBP-1c, FAS, and ACC and increased expression of PPARα, ATGL, and LPL. GSPE treatment for 1 h significantly decreased expression of pro-inflammatory cytokines (TNFα, IL-6, IL-1β, and NF-κB) and increased the expression of anti-inflammatory cytokines (IL-10 and TGF-β1). In vivo, the administration of GSPE significantly reduced high-fat diet-induced increase of serum CHOL, TG, and HDL, but increased LDL content. GSPE treatment for 3 h increased expression of ATGL and LPL, and significantly decreased the expression of HFD-fed-induced SREBP-1c, ACC, FAS, PPARγ, PPARα, and H-FABP. GSPE treatment for 3 h also significantly decreased the expression of pro-inflammatory cytokines (TNFα, IL-6, and IL-1β) and increased the expression of the anti-inflammatory cytokine IL-10. The expression levels of the lipogenic miRNAs, miR-33, and miR-122, were suppressed both in vivo and in vitro by GSPE. In summary, GSPE had hypolipidemic and potential anti-inflammatory effects in the liver, potentially mediated by miR-33 and miR-122.

中文翻译:

葡萄籽原花青素提取物可改善草鱼(Ctenopharyngodon idella)中肝脂质的积累和炎症。

由于鱼类中脂肪过多积累引起的肝脂质代谢紊乱是水产养殖中的重要问题。研究表明,葡萄籽原花青素提取物(GSPE)可以调节鱼的脂质代谢并提高鱼的免疫力。但是,机制尚不清楚。在这项研究中,我们以草鱼为模型,草鱼将多余的脂肪储存在肝脏中。在体外,GSPE处理肝细胞3小时可显着降低TG含量,并伴有SREBP-1c,FAS和ACC的表达降低以及PPARα,ATGL和LPL的表达增加。GSPE处理1小时可显着降低促炎细胞因子(TNFα,IL-6,IL-1β和NF-κB)的表达,并增加抗炎细胞因子(IL-10和TGF-β1)的表达。在体内,GSPE的使用可显着降低高脂饮食诱导的血清CHOL升高,TG和HDL,但LDL含量增加。GSPE处理3小时可增加ATGL和LPL的表达,并显着降低HFD喂养的SREBP-1c,ACC,FAS,PPARγ,PPARα和H-FABP的表达。GSPE处理3小时还显着降低了促炎细胞因子(TNFα,IL-6和IL-1β)的表达,并增加了抗炎细胞因子IL-10的表达。GSPE在体内和体外均抑制了致脂性miRNA,miR-33和miR-122的表达水平。总之,GSPE在肝脏中具有降血脂和潜在的抗炎作用,可能由miR-33和miR-122介导。GSPE处理3小时还显着降低了促炎细胞因子(TNFα,IL-6和IL-1β)的表达,并增加了抗炎细胞因子IL-10的表达。GSPE在体内和体外均抑制了致脂性miRNA,miR-33和miR-122的表达水平。总之,GSPE在肝脏中具有降血脂和潜在的抗炎作用,可能由miR-33和miR-122介导。GSPE处理3 h​​还显着降低了促炎细胞因子(TNFα,IL-6和IL-1β)的表达,并增加了抗炎细胞因子IL-10的表达。GSPE在体内和体外均抑制了致脂性miRNA,miR-33和miR-122的表达水平。总之,GSPE在肝脏中具有降血脂和潜在的抗炎作用,可能是由miR-33和miR-122介导的。
更新日期:2020-05-24
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